Abstract
Graft copolymers consisting of poly(N-isopropylacrylamide) and single-stranded DNA were found to form DNA-carrying nanoparticles above physiological temperature. Aggregation of the nanoparticles was induced at relatively high salt concentration by the hybridization of surface-anchored DNA with full-match complementary DNA. In contrast, single-base mismatches at the distal end stabilized the colloidal dispersion so that the dispersion remained transparent. The phenomenon is applicable to SNP typing.
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Maeda, M. Sequence-Specific Aggregation Behavior of DNA-Carrying Colloidal Nanoparticles Prepared from Poly(N-isopropylacrylamide)-graft-Oligodeoxyribonucleotide. Polym J 38, 1099–1104 (2006). https://doi.org/10.1295/polymj.PJ2006107
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DOI: https://doi.org/10.1295/polymj.PJ2006107