Abstract
Chondroitin sulfate (CS) as a sodium salt was impregnated within micropores of silica gel on a thin-layer chromatographic plate and coated by a thin film of plasma-polymerized propargyl alcohol (PPPA) for immobilization of CS macromolecules. Chromatographic behavior of cations such as Co2+, Fe2+, and Cu2+ was observed on the prepared thin-layer plate to explore ion-exchange functions from the acidic groups of CS. Affinity of the cations to the immobilized CS plate was enhanced with increasing CS impregnated in the silica gel, regardless of the kind of cation. Moreover, affinity was significantly altered by the mode of preliminary washing of the thin-layer plate, use of either aqueous or organic solvent, and adjusted pH of the solvent. The PPPA film itself indicated certain affinity to the cations, likely due to the oxygen-containing groups, e.g., -OH, partly dissociable in the polymer matrix. The proposed technique suggests other new thin-layer chromatographic plates in which various ion-attractive or ion-exchange materials are immobilized.
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Yoshimura, K., Horita, T. & Hozumi, K. Thin-Layer Chromatography of Metal Ions on Immobilized Chondroitin Sulfate Plate Prepared by Plasma Polymerization Technique. Polym J 28, 261–265 (1996). https://doi.org/10.1295/polymj.28.261
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DOI: https://doi.org/10.1295/polymj.28.261