Abstract
The heats of mixing for an equimolar mixture of poly(A) and poly(U) in buffer solutions of various concentrations were measured at 298.15±0.005 K by calorimetry. The apparent heats of mixing obtained depended on buffer concentration. The degree of formation of the poly(A)·poly(U) duplex and fraction of stacking of poly(A) in various buffer concentrations were also estimated from CD spectral measurements. By a combination of the results of calorimetric and spectral methods, the net heat of formation of poly(A)·poly(U) duplex with high ordered structure from random coil conformation of constituent polynucleotide was estimated to be about −67.2 kJ (bpm)−1, using the reaction cycle.
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Tarui, H., Baba, Y., Tada, K. et al. Interaction between Polynucleotides VI. Ionic Strength Dependence of the Poly(A) ·Poly(U) Duplex Formation. Polym J 18, 379–384 (1986). https://doi.org/10.1295/polymj.18.379
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DOI: https://doi.org/10.1295/polymj.18.379