Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

NMR disentangles a dynamic disaggregase machinery

Nature Structural & Molecular Biology volume 20, pages 409–410 (2013)Cite this article

Subjects

The three-dimensional structure of a key complex in chaperone-mediated protein disaggregation, comprising ClpB and DnaK, has been determined using NMR. In addition to providing unique mechanistic insights, the approach promises to elucidate the structural basis for the assembly of elusive dynamic protein machineries in the near future.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Chaperones and cofactors involved in the ClpB disaggregase machinery.

References

  1. Hartl, F.U., Bracher, A. & Hayer-Hartl, M. Nature 475, 324–332 (2011).

    Article  CAS  Google Scholar 

  2. Hartl, F.U. & Hayer-Hartl, M. Nat. Struct. Mol. Biol. 16, 574–581 (2009).

    Article  CAS  Google Scholar 

  3. Tyedmers, J., Mogk, A. & Bukau, B. Nat. Rev. Mol. Cell Biol. 11, 777–788 (2010).

    Article  CAS  Google Scholar 

  4. Doyle, S.M. & Wickner, S. Trends Biochem. Sci. 34, 40–48 (2009).

    Article  CAS  Google Scholar 

  5. Harrison, C.J., Hayer-Hartl, M., Di Liberto, M., Hartl, F. & Kuriyan, J. Science 276, 431–435 (1997).

    Article  CAS  Google Scholar 

  6. Zhu, X. et al. Science 272, 1606–1614 (1996).

    Article  CAS  Google Scholar 

  7. Lee, S. et al. Cell 115, 229–240 (2003).

    Article  CAS  Google Scholar 

  8. Rosenzweig, R., Moradi, S., Zarrine-Afsar, A., Glover, J.R. & Kay, L.E. Science 339, 1080–1083 (2013).

    Article  CAS  Google Scholar 

  9. Sprangers, R. & Kay, L.E. Nature 445, 618–622 (2007).

    Article  CAS  Google Scholar 

  10. Ruschak, A.M. & Kay, L.E. Proc. Natl. Acad. Sci. USA 109, E3454–E3462 (2012).

    Article  CAS  Google Scholar 

  11. Religa, T.L., Sprangers, R. & Kay, L.E. Science 328, 98–102 (2010).

    Article  CAS  Google Scholar 

  12. Gelis, I. et al. Cell 131, 756–769 (2007).

    Article  CAS  Google Scholar 

  13. Zhuravleva, A., Clérico, E.M. & Gierasch, L.M. Cell 151, 1296–1307 (2012).

    Article  CAS  Google Scholar 

  14. Hamel, D.J. & Dahlquist, F. J. Am. Chem. Soc. 127, 9676–9677 (2005).

    Article  CAS  Google Scholar 

  15. Takahashi, H. et al. J. Biomol. NMR 34, 167–177 (2006).

    Article  CAS  Google Scholar 

  16. Chen, L. et al. Cell Rep. doi:10.1016/j.celrep.2013.02.025 (in the press).

  17. Battiste, J.L. & Wagner, G. Biochemistry 39, 5355–5365 (2000).

    Article  CAS  Google Scholar 

  18. Clore, G.M., Tang, C. & Iwahara, J. Curr. Opin. Struct. Biol. 17, 603–616 (2007).

    Article  CAS  Google Scholar 

  19. Wendler, P. et al. Cell 131, 1366–1377 (2007).

    Article  CAS  Google Scholar 

  20. Saibil, H.R. Science 339, 1040–1041 (2013).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Tomohide Saio and Charalampos G. Kalodimos are at the Center for Integrative Proteomics Research and Department of Chemistry & Chemical Biology, Rutgers University, Piscataway, New Jersey, USA.,

    Tomohide Saio & Charalampos G Kalodimos

Authors
  1. Tomohide Saio
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Charalampos G Kalodimos
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Charalampos G Kalodimos.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Saio, T., Kalodimos, C. NMR disentangles a dynamic disaggregase machinery. Nat Struct Mol Biol 20, 409–410 (2013). https://doi.org/10.1038/nsmb.2551

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nsmb.2551

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing