Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Correspondence
  • Published:

Mg2+–Mn2+ clusters in enzyme-catalyzed phosphoryl-transfer reactions

Here we show how Mg2+ and Mn2+ function in Escherichia coli phosphoenolpyruvate carboxykinase (PCK) and we propose a general model for the role of binuclear metal clusters in enzyme-catalyzed phosphoryl-transfer reactions.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

References

  1. Utter, M.F. & Kolenbrander, H.M. In The Enzymes (ed. Boyer, P. D.) 117–168 (Academic Press, NY; 1972).

    Google Scholar 

  2. Matte, A., Tari, L.W., Goldie, H. & Delbaere, L.T.J. J. Biol. Chem. 272, 8105–8108 (1997).

    Article  CAS  Google Scholar 

  3. Dismukes, G.C. Chem. Rev., 96, 2909–2926 (1996).

    Article  CAS  Google Scholar 

  4. Goldie, A.H. & Sanwal, B.D. J. Biol. Chem. 255, 1399–1405 (1980).

    CAS  PubMed  Google Scholar 

  5. Lee, M.H., Hebda, C.A. & Nowak, T. J. Biol. Chem. 256, 12793–12801 (1981).

    CAS  PubMed  Google Scholar 

  6. Rohrer, S.P., Saz, H.J. & Nowak, T. Biol. Chem. 261, 13049–13055 (1986).

    CAS  Google Scholar 

  7. Maggini, S., Stoecklin-Tschan, F.B., Mörikofer-Zwez, S. & Walter, P. Biochem. J. 292, 365–370 (1993).

    Article  CAS  Google Scholar 

  8. Hebda, C.A. & Nowak, T. J. Biol. Chem. 257, 5515–5522 (1982).

    CAS  PubMed  Google Scholar 

  9. Ramakrishnan, C. & Ramachadran, G.N. Biophys. J. 5, 909–933 (1965).

    Article  CAS  Google Scholar 

  10. Matte, A., Goldie, H., Sweet, R.M. & Delbaere, L.T.J. J. Mol. Biol. 256, 126–143 (1996).

    Article  CAS  Google Scholar 

  11. Tari, L.W., Matte, A., Pugazenthi, U., Goldie, H. & Delbaere, L.T.J. Nature Struct. Biol. 3, 355–363 (1996).

    Article  CAS  Google Scholar 

  12. Williams, R.J.P. In Magnesium and the Cell (ed. Birch, N. J.) 15–30 (Academic Press, London; 1993).

    Google Scholar 

  13. Matte, A. Ph. D. thesis, University of Saskatchewan, Saskatoon, Canada (1996).

  14. Tarn, S. -C. & Williams, R. J. P. Struct. Bond 63, 103–151 (1985).

    Article  Google Scholar 

  15. Fraústo da Silva, J. J. R. & Williams, R. J. P. . In The Biological Chemistry of the Elements: The Inorganic Chemistry of Life, 30–40 (Clarendon Press, Oxford; 1991).

    Google Scholar 

  16. Berchtold, H. et al. Nature 365, 126–132 (1993)

    Article  CAS  Google Scholar 

  17. Wigley, D.B. et al. Nature 351, 624–629 (1991).

    Article  CAS  Google Scholar 

  18. Pai, E.F. et al. Nature 341, 209–214 (1989).

    Article  CAS  Google Scholar 

  19. Colombo, G. & Lardy, H.A. Biochemistry 20, 2758–2767 (1981).

    Article  CAS  Google Scholar 

  20. Lee, M.H. & Nowak, T. Biochemistry 23, 6506–6513 (1984).

    Article  CAS  Google Scholar 

  21. Duffy, T.H. & Nowak, T. Biochemistry 24, 1152–1160 (1985).

    Article  CAS  Google Scholar 

  22. Hlavaty, J.J. & Nowak, T. Biochemistry 36, 3389–3403 (1997).

    Article  CAS  Google Scholar 

  23. Sigel, H. et al. J. Am. Chem. Soc. 106, 7935–7946 (1984).

    Article  CAS  Google Scholar 

  24. Sigel, H. Coord. Chem. Rev. 100, 453–539 (1990).

    Article  CAS  Google Scholar 

  25. Baek, Y.H. & Nowak, T. Arch. Biochem. Biophys. 217, 491–197 (1982).

    Article  CAS  Google Scholar 

  26. Frick, D.N. et al. J. Biol, Chem. 269, 1794–1803 (1994).

    CAS  Google Scholar 

  27. Hunt, J.B. et al. Arch. Biochem. Biophys. 166, 102–124 (1975).

    Article  CAS  Google Scholar 

  28. Balakrishnan, M.S. & Villafranca, J.J. Biochemistry 17, 3531–3538 (1978).

    Article  CAS  Google Scholar 

  29. Liaw, S-H. & Eisenberg, D. Biochemistry 33, 675–681 (1994).

    Article  CAS  Google Scholar 

  30. Lodato, D.T. & Reed, G.H. Biochemistry 26, 2243–2250 (1987).

    Article  CAS  Google Scholar 

  31. Buchbinder, J.L. & Reed, G.H. Biochemistry 29, 1799–1806 (1990).

    Article  CAS  Google Scholar 

  32. Fraústo da Silva, J. J. R. & Williams, R. J. P. . In The biological chemistry of the elements: the inorganic chem-istry of life, 247 (Clarendon Press, Oxford; 1991).

    Google Scholar 

  33. Collaborative Computational Project ♯ 4, Acta Crys-tallogr. D50, 760–763 (1994).

  34. French, S. & Wilson, K. Acta Crystallogr. A34 517–525 (1978).

    Article  CAS  Google Scholar 

  35. Brünger, A.T., Kuriyan, J. & Karplus, M. Science 235, 458–460 (1987).

    Article  Google Scholar 

  36. Roussel, A. & Cambillau, C. TURBO FRODO. In Silicon Graphics Geometry Partner Directory, 77–88 (Silicon Graphics, Mountain View, California; 1989).

    Google Scholar 

  37. Evans, S.V. J. Mol. Graphics 11, 134–138 (1993).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tari, L., Matte, A., Goldie, H. et al. Mg2+–Mn2+ clusters in enzyme-catalyzed phosphoryl-transfer reactions. Nat Struct Mol Biol 4, 990–994 (1997). https://doi.org/10.1038/nsb1297-990

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nsb1297-990

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing