Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Correspondence
  • Published:

A passive transmembrane helix

Nature Structural Biology volume 4, pages 986–990 (1997)Cite this article

Every residue of a putative transmembrane helix in diacylglycerol kinase can be converted to both alanine and leucine while maintaining high specific activity, indicating that some transmembrane helices play a relatively passive role in structure and function.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

References

  1. Chang, C., El-Kabbani, O., Tiede, D., Norris, J. & Shifter, M. Biochemistry 30, 5352–5369 (1991).

    Article  CAS  Google Scholar 

  2. Deisenhofer, J. & Michel, H. EMBO J. 8, 2149–2169 (1989).

    Article  CAS  Google Scholar 

  3. Engelman, D., Steitz, T. & Goldman, A. Ann. Rev. Biophys. Biophys. Chem. 15, 321–353 (1986).

    Article  CAS  Google Scholar 

  4. Henderson, R. et al. J. Mol. Biol. 213, 899–929 (1990).

    Article  CAS  Google Scholar 

  5. Rees, D., Komiya, H., Yeates, T., Allen, J. & Feher, G. Annu. Rev. Biochem. 58, 607–633 (1989).

    Article  CAS  Google Scholar 

  6. Tsukihara, T. et al. Science 272, 1136–1144 (1996).

    Article  CAS  Google Scholar 

  7. Grigorieff, N., Ceska, T., Downing, K., Baldwin, J. & Henderson, R. J. Mol. Biol. 259, 393–421 (1996).

    Article  CAS  Google Scholar 

  8. Iwata, S., Ostermeier, C., Ludwig, B. & Michel, H. Nature 376, 660–669 (1995).

    Article  CAS  Google Scholar 

  9. MacKenzie, K., Prestegard, J. & Engelman, D. Science 276, 131–133 (1997).

    Article  CAS  Google Scholar 

  10. Popot, J. & Engelman, D. Biochemistry 29, 4031–4037 (1990).

    Article  CAS  Google Scholar 

  11. Lemmon, M. et al. J. Biol. Chem. 267, 7683 (1992).

    CAS  PubMed  Google Scholar 

  12. Lemmon, M., Flanagan, J., Treutlein, H., Zhang, J. & Engelman, D. Biochemistry 31, 12719–12725 (1992).

    Article  CAS  Google Scholar 

  13. Kahn, T. & Engelman, D. Biochemistry 31, 6144–6151 (1992).

    Article  CAS  Google Scholar 

  14. Liao, M. J., London, E. & Khorana, H. G. J Biol Chem 258, 9949–9955 (1983).

    CAS  PubMed  Google Scholar 

  15. Bibi, E. & Kaback, H. Proc. Natl. Acad. Sci., USA 87, 4325–4329 (1990).

    Article  CAS  Google Scholar 

  16. Wrubel, W., Stochaj, U., Sonnewald, U., Theres, C. & Ehring, R. J. Bacteriol, 172, 5374–5381 (1990).

    Article  CAS  Google Scholar 

  17. Haltia, T. & Freire, E. Biochim. Biophys. Acta 1228, 1–27 (1995).

    Article  Google Scholar 

  18. Kahn, T., Sturtevant, J. & Engelman, D. Biochemistry 31, 8829–8839.

  19. Loomis, C., Walsh, J. & Bell, R. J. Biol. Chem. 260, 4091–4097 (1985).

    CAS  PubMed  Google Scholar 

  20. Lightner, V., Bell, R. & Modrich, P. J. Biol. Chem. 258, 10856–10861 (1983).

    CAS  PubMed  Google Scholar 

  21. Smith, R., O'Toole, J., Maguire, M. & Sanders, C. J. Bacteriol. 176, 5459–5465 (1994).

    Article  CAS  Google Scholar 

  22. Sanders, C. R. et al. Biochemistry 35, 8610–8618 (1996).

    Article  CAS  Google Scholar 

  23. Wen, J., Chen, X. & Bowie, J. Nature Struct. Biol. 3, 141–148 (1996).

    Article  CAS  Google Scholar 

  24. Guzman, L., Weiss, D. & Beckwith, J. J. Bact. in the press (1997).

  25. Pohlschroder, M., Murphy, C. & Beckwith, J. J. Biol. Chem. 271, 19908–19914 (1996).

    Article  CAS  Google Scholar 

  26. Deber, C. & Li, S. Biopolymers 37, 295–318 (1995).

    Article  CAS  Google Scholar 

  27. Chuang, H. & Bell, F. Arch. Bioch. Biophys. 152, 502–514 (1972).

    Article  CAS  Google Scholar 

  28. Tomazic, S. & Klibanov, A. J. Biol. Chem. 263, 3092–3096 (1988).

    CAS  PubMed  Google Scholar 

  29. Pace, C. & McGrath, T. J. Biol. Chem. 255, 3862–3865 (1980).

    CAS  PubMed  Google Scholar 

  30. Axelsen, P., Kaufman, B., McElhaney, R. & Lewis, R. Biophys. J. 69, 2770–2781 (1995).

    Article  CAS  Google Scholar 

  31. Bowie, J. U. J. Mol. Biol. 272, 780–789 (1997).

    Article  CAS  Google Scholar 

  32. Langosch, D., Bosig, B., Kolmar, H. & Fritz, H. J. Mol. Biol. 263, 525–530 (1996).

    Article  CAS  Google Scholar 

  33. Lemmon, M. & Engelman, D. Curr. Op. Struct.Biol. 2, 511–518 (1992).

    Article  CAS  Google Scholar 

  34. Desjarlais, J. & Handel, T. Curr. Opin. Biotech. 6, 460–466 (1995).

    Article  CAS  Google Scholar 

  35. Lemmon, M. A. & Engelman, D. M. Q. Rev. Biophys. 27, 157–218 (1994).

    Article  CAS  Google Scholar 

  36. Lau, F. & Bowie, J. Biochemistry in the press (1997).

  37. Miller, K., McKinstry, M., Hunt, W. & Nixon, B. Mol. Plant-Microbe Int. 5, 363–371 (1992).

    Article  CAS  Google Scholar 

  38. Walsh, J. & Bell, R. J. Biol. Chem. 261, 6239–6247 (1986).

    CAS  PubMed  Google Scholar 

  39. Raetz, C. & Newman, K. J. Biol. Chem. 253, 3882–3887 (1978).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry and Biochemistry and Laboratory of Structural Biology and Molecular Medicine, University of California, Los Angeles, Los Angeles, California, 90095, USA

    Yufeng Zhou, Juan Wen & James U. Bowie

Authors
  1. Yufeng Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Juan Wen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. James U. Bowie
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhou, Y., Wen, J. & Bowie, J. A passive transmembrane helix. Nat Struct Mol Biol 4, 986–990 (1997). https://doi.org/10.1038/nsb1297-986

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nsb1297-986

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing