Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Correspondence
  • Published:

Dimerization of the UmuD' protein in solution and its implications for regulation of SOS mutagenesis

Nature Structural Biology volume 4, pages 979–982 (1997)Cite this article

NMR spectroscopy has been used to determine that the dimerization interface of UmuD' in solution is not the homodimer interface originally inferred from crystallographic data. Instead, it resembles an interface that had been hypothesized to be involved in filamentation.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

References

  1. Friedberg, E.C., Walker, G.C. & Siede, W. DNA Repair and Mutagenesis (American Society for Microbiology Press, Washington D.C., 1995).

    Google Scholar 

  2. Rajagopalan, M. et al. Proc. Natl. Acad. Sci. USA 89, 10777–10781 (1992).

    Article  CAS  Google Scholar 

  3. Woodgate, R. & Sedgwick, S. Mol. Microbiol. 6, 2213–2218 (1992).

    Article  CAS  Google Scholar 

  4. Nohmi, T, Battista, J.R., Dodson, L.A. & Walker, G.C. Proc. Natl. Acad. Sci. USA 85, 1816–1820 (1988).

    Article  CAS  Google Scholar 

  5. Little, J.W. J. Bacteriol. 175, 4943–4950 (1993).

    Article  CAS  Google Scholar 

  6. Battista, J.R., Ohta, T., Nohmi, T., Sun, W. & Walker, G.C. Proc. Natl. Acad. Sci. USA 87, 7190–7194 (1990).

    Article  CAS  Google Scholar 

  7. Peat, T.S. et al. Nature 380, 727–730 (1996).

    Article  CAS  Google Scholar 

  8. Peat, T.S. et al. Structure 4, 1401–1412 (1996).

    Article  CAS  Google Scholar 

  9. Walters, K.J., Matsuo, H. & Wagner, G. J. Am. Chem. Soc. 119, 5958–5959 (1996).

    Article  Google Scholar 

  10. Talluri, S. & Wagner, G. J. Magn. Res. B 112, 200–205 (1996).

    Article  CAS  Google Scholar 

  11. Lee, M.H., Guzzo, A. & Walker, G.C. J. Bacteriol. 178, 7304–7307 (1996).

    Article  CAS  Google Scholar 

  12. Slilaty, S.N., Rupley, J.A. & Little, J.W. Biochemistry 25, 6866–6875 (1986).

    Article  CAS  Google Scholar 

  13. Phizicky, E.M. & Roberts, J.W. J. Mol. Biol. 139, 319–328 (1980).

    Article  CAS  Google Scholar 

  14. Cohen, S., Knoll, B.J., Little, J.W. & Mount, D.W. Nature 294, 182–184 (1981).

    Article  CAS  Google Scholar 

  15. Gimble, F.S. & Sauer, R.T. J. Mol. Biol. 206, 29–39 (1989).

    Article  CAS  Google Scholar 

  16. Lee, M.H., Ohta, T. & Walker, G.C. J. Bacteriol. 176, 4825–4837 (1994).

    Article  CAS  Google Scholar 

  17. Guzzo, A., Lee, M.H., Oda, K. & Walker, G.C. J. Bacteriol. 178, 7295–7303 (1994).

    Article  Google Scholar 

  18. Burz, D.S., Beckett, D., Benson, N. & Ackers, G.K. Biochemistry 33, 8399–8405 (1994).

    Article  CAS  Google Scholar 

  19. Carlson, P.A. & Koudelka, G.B. J. Bacteriol. 176, 6907–6914 (1994).

    Article  CAS  Google Scholar 

  20. Jordan, S. & Pabo, C. Science 242, 893–899 (1988).

    Article  CAS  Google Scholar 

  21. Aggarwal, A., Rodgers, D., Drottar, M., Ptashne, M. & Harrison, S. Science 242, 899–907 (1988).

    Article  CAS  Google Scholar 

  22. Anderson, J., Ptashne, M. & Harrison, S. Nature 326, 846–852 (1987).

    Article  CAS  Google Scholar 

  23. Jonczyk, P. & Nowicka, A. J. Bacteriol. 178, 2580–2585 (1996).

    Article  CAS  Google Scholar 

  24. Studier, F.W., Rosenberg, A.H., Dunn, J.J. & Dubendorff, J.W. Meth. Enz. 185, 60–89 (1990).

    Article  CAS  Google Scholar 

  25. Looman, A.C. et al. EMBO J. 6, 2489–2492 (1987).

    Article  CAS  Google Scholar 

  26. Piotto, M., Saudek, V. & Skenar, V. J. Biomol. NMR 2, 661–665 (1992).

    Article  CAS  Google Scholar 

  27. Dayie, K.T. & Wagner, G. J. Magn. Res. A 111, 121–126 (1994).

    Article  CAS  Google Scholar 

  28. Peng, J.W. & Wagner, G. J. Magn. Res. 98, 308–332 (1992).

    CAS  Google Scholar 

  29. Markus, M.A., Dayie, K.T., Matsudaira, P. & Wagner, G. Biochemistry 35, 1722–1732 (1996).

    Article  CAS  Google Scholar 

  30. Kay, L.E., Torchia, D. & Bax, A. Biochemistry 28, 8972–8979 (1989).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, 02115, USA

    Ann E. Ferentz & Gerhard Wagner

  2. Biology Department, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA

    Timothy Opperman & Graham C. Walker

Authors
  1. Ann E. Ferentz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Timothy Opperman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Graham C. Walker
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Gerhard Wagner
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ferentz, A., Opperman, T., Walker, G. et al. Dimerization of the UmuD' protein in solution and its implications for regulation of SOS mutagenesis. Nat Struct Mol Biol 4, 979–982 (1997). https://doi.org/10.1038/nsb1297-979

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nsb1297-979

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing