Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Correspondence
  • Published:

The CHIP28 water channel visualized in ice by electron crystallography

Nature Structural Biology volume 2, pages 726–729 (1995)Cite this article

Electron crystallography of frozen-hydrated two-dimensional crystals of deglycosylated human erythrocyte CHIP28 reveals an aqueous vestibule in each monomer leading to the water-selective channel that is enclosed by multiple transmembrane α-helices.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

References

  1. Agre, P. et al. Am. J. Physiol. 265, F463–476 (1993).

    CAS  PubMed  Google Scholar 

  2. Verkman, A.S. Water channels (R.G. Landes Company, Austin, Texas 1993).

    Google Scholar 

  3. Knepper, M.A. Proc. natn. Acad. Sci. U.S.A. 91, 6255–6258 (1994).

    Article  CAS  Google Scholar 

  4. Gorin, M.B., Yancey, S.B., Cline, J., Revel, J.-P., & Horwitz, J.P. Cell 39, 49–59 (1984).

    Article  CAS  Google Scholar 

  5. Pao, G.M. et al. Mol. Microbiol. 5, 33–37 (1991).

    Article  CAS  Google Scholar 

  6. Preston, G.M. & Agre, P. Proc. natn. Acad. Sci. U.S.A. 88, 11110–11114 (1991).

    Article  CAS  Google Scholar 

  7. Van Hoek, A.N. et al. Biochemistry 32, 11847–11856 (1993).

    Article  CAS  Google Scholar 

  8. Van Hoek, A.N. et al. Biochemistry 34, 2212–2219 (1995).

    Article  CAS  Google Scholar 

  9. Preston, G.M., Jung, J.S., Guggino, W.B. & Agre, P. J. biol. Chem. 268, 17–20 (1993).

    CAS  PubMed  Google Scholar 

  10. Verbavatz, J.-M. et al. J. Cell Biol. 123, 605–618 (1993).

    Article  CAS  Google Scholar 

  11. Mitra, A.K., Yeager, M., van Hoek, A.N., Wiener, M.C., & Verkman, A.S. Biochemistry 33, 12735–12740 (1994).

    Article  CAS  Google Scholar 

  12. Walz, T., Smith, B.L., Zeidel, M., Engel, A., & Agre, P. J. biol. Chem. 269, 1583–1586 (1994).

    CAS  PubMed  Google Scholar 

  13. Walz, T., Smith, B.L., Agre, P., & Engel, A. EMBO J. 13, 2985–2993 (1994).

    Article  CAS  Google Scholar 

  14. Van Hoek, A.N. et al. J. biol. Chem. 266, 16633–16635 (1991).

    CAS  PubMed  Google Scholar 

  15. Shi, L.-b., Skach, W.R., & Verkman, A.S. J. biol. Chem. 269, 10417–10422 (1994).

    CAS  PubMed  Google Scholar 

  16. Preston, G.M., Jung, J.S., Guggino, W.B. & Agre, P. J. biol. Chem. 269, 1668–1673 (1994).

    CAS  PubMed  Google Scholar 

  17. Skach, W.R. et al. J. Cell Biol. 125, 803–815 (1994).

    Article  CAS  Google Scholar 

  18. Henderson, R., Baldwin, J.M., Ceska, T.A., Zemlin, F., Beckmann, E. & Downing, K.H. J. molec. Biol. 213, 899–929 (1990).

    Article  CAS  Google Scholar 

  19. Mitra, A.K., & Stroud, R.M. Biophys. J. 57, 301–311 (1990).

    Article  CAS  Google Scholar 

  20. Mitra, A.K., Miercke, L.J.W., Turner, G.J., Shand, R.F., Betlach, M.C., & Stroud, R.M. Biophys. J. 65, 1295–1306 (1993).

    Article  CAS  Google Scholar 

  21. Dickerson, R.E., Weinzierl, J.E. & Palmer, R.A. Acta Crystallogr. 24, 997–1003 (1968).

    Article  CAS  Google Scholar 

  22. Baldwin, J.M., Henderson, R., Beckman, E., & Zemlin, F. J. molec. Biol. 202, 585–591 (1988).

    Article  CAS  Google Scholar 

  23. Unwin, P.N.T. & Henderson, R. J. molec. Biol. 94, 425–440 (1975).

    Article  CAS  Google Scholar 

  24. Havelka, W.A., Henderson, R., Heymann, J.A.W. & Oesterhelt, D. J. molec. Biol. 234, 837–846 (1994).

    Article  Google Scholar 

  25. Jap, B., Maestre, M.F., Hayward, S.B., & Glaeser, R.M. Biophys. J. 43, 81–89 (1983).

    Article  CAS  Google Scholar 

  26. Jap, B.K. J. molec. Biol. 205, 407–419 (1989).

    Article  CAS  Google Scholar 

  27. Kleffel, B., Garavito, R.M., Baumeister, W. & Rosenbusch, J.P. EMBO J. 4, 1589–1592 (1985).

    Article  CAS  Google Scholar 

  28. Fischbarg, J., Li, J., Cheung, M., Czegledy, F., Iserovich, P. & Kuang, K. J. Membrane Biol. 143, 177–188 (1995).

    Article  CAS  Google Scholar 

  29. Jap, B.K., Walian, P.J. & Gehring, K. Nature 350, 167–170 (1991).

    Article  CAS  Google Scholar 

  30. Mannella, C.A., Forte, M. & Colombini, M. J. bioenerg. Biomembranes 24, 7–19 (1992).

    Article  CAS  Google Scholar 

  31. Unwin, P.N.T. & Ennis, P.D. Nature 307, 609–613 (1984).

    Article  CAS  Google Scholar 

  32. Sather, W.A., Yang, J., & Tsien, R.W. Curr. Opin. Neurobiol. 4, 313–323 (1994).

    Article  CAS  Google Scholar 

  33. Unwin, N. J. molec. Biol. 229, 1101–1124 (1993).

    Article  CAS  Google Scholar 

  34. Kühlbrandt, W., Wang, D.N., Fujiyoshi, Y. Nature 367, 614–621 (1994).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cell Biology, The Scripps Research Institute, 10666 North Torrey Pines Road, La Jolla, California, 92037, USA

    A.K. Mitra & M. Yeager

  2. Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California San Francisco, San Francisco, California, 94143-0521, USA

    A.N. van Hoek & A.S. Verkman

  3. Department of Biophysics and Biochemistry, University of California San Francisco, San Francisco, California, 94143-0448, USA

    M.C. Wiener

  4. Division of Cardiovascular Diseases, Scripps Clinic and Research Foundation, 10666 North Torrey Pines Road, La Jolla, California, 92037, USA

    M. Yeager

Authors
  1. A.K. Mitra
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A.N. van Hoek
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M.C. Wiener
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A.S. Verkman
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Yeager
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mitra, A., van Hoek, A., Wiener, M. et al. The CHIP28 water channel visualized in ice by electron crystallography. Nat Struct Mol Biol 2, 726–729 (1995). https://doi.org/10.1038/nsb0995-726

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nsb0995-726

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing