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Electron cryomicroscopy and angular reconstitution used to visualize the skeletal muscle calcium release channel

Nature Structural Biology volume 2, pages 18–24 (1995)Cite this article

Abstract

We exploit the random orientations of ice-embedded molecules imaged in an electron cryomicroscope to determine the three-dimensional structure of the Ca2+-release channel from the sarcoplasmic reticulum (SR) in its closed state, without tilting the specimen holder. Our new reconstruction approach includes an exhaustive search of all different characteristic projection images in the micrographs and the assignment ofEuler angle orientations to these views. The 30 Å map implied reveals a structure in which the transmembrane region exhibits no apparent opening on the SR lumen side. The extended cytoplasmic region has a hollow appearance and consists, in each monomer, of a clamp-shaped and a handle-shaped domain.

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Authors and Affiliations

  1. Verna and Marrs McLean Department of Biochemistry and The W. M. Keck Center for Computational Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas, 77030, USA

    Irina I. Serysheva, Elena V. Orlova, Wah Chiu & Michael B. Sherman

  2. Department of Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas, 77030, USA

    Irina I. Serysheva, Wah Chiu & Susan L. Hamilton

  3. Fritz Haber Institute of the Max Planck Society, Faradayweg 4-6, D-14195, Berlin, Germany

    Elena V. Orlova & Marin van Heel

Authors
  1. Irina I. Serysheva
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  2. Elena V. Orlova
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  3. Wah Chiu
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  4. Michael B. Sherman
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  5. Susan L. Hamilton
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  6. Marin van Heel
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Serysheva, I., Orlova, E., Chiu, W. et al. Electron cryomicroscopy and angular reconstitution used to visualize the skeletal muscle calcium release channel. Nat Struct Mol Biol 2, 18–24 (1995). https://doi.org/10.1038/nsb0195-18

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  • DOI: https://doi.org/10.1038/nsb0195-18

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