Abstract
Pro- and mature BDNF activate very different receptors and intracellular pathways, potentially leading to either neuronal death or survival. Here we examined the biochemistry of endogenous BDNF in mouse neurons using sensitive reagents and found that pro-BDNF is rapidly converted intracellularly to mature BDNF, the latter being stored and released by excitatory input.
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Acknowledgements
We thank S. Wiese and M. Sendtner for the floxed BDNF line and the BDNF antibodies, B. Fayard for constructs, and V. Crotet for technical assistance. T.M. was supported by the Uehara Memorial Foundation and by the Mochida Memorial Foundation for Medical and Pharmaceutical Research and M.K. by the Deutsche Forschungsgemeinschaft.
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S.R. generated the cBdnf mutant mice. J.K. and R.K. generated the Bdnf-Myc mutant mice. M.P. and M.K. carried out the electrophysiology. T.M. and Y.-A.B. designed and carried out all of the other experiments, and wrote the manuscript.
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Matsumoto, T., Rauskolb, S., Polack, M. et al. Biosynthesis and processing of endogenous BDNF: CNS neurons store and secrete BDNF, not pro-BDNF. Nat Neurosci 11, 131–133 (2008). https://doi.org/10.1038/nn2038
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DOI: https://doi.org/10.1038/nn2038
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