Abstract
Commissural axons cross the ventral midline of the neural tube in a Slit-dependent manner. The underlying molecular mechanisms remain unclear. We found that the deubiquitinating enzyme USP33 interacts with the Robo1 receptor. USP33 was essential for midline crossing by commissural axons and for their response to Slit. Our results reveal a previously unknown role for USP33 in vertebrate commissural axon guidance and in Slit signaling.
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Acknowledgements
We thank Y. Zou and F. Murakami for generously providing the Sema3F construct and the antibody to Robo1, respectively, M. Katakura for support during this work and X. Chen for excellent technical support. This work has been supported by the James S. McDonnell Foundation (grant JSMF 220020180 to J.Y.W.) and the US National Institutes of Health (grants CA114197 and CA107193 to J.Y.W.).
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J.Y.-K. and M.K.-K. carried out the experiments. J.Y.-K., M.K.-K., J.Y.W. and Y.R. designed the experiments, analyzed the data and wrote the manuscript. G.W. provided the GFP-USP33 construct.
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Yuasa-Kawada, J., Kinoshita-Kawada, M., Wu, G. et al. Midline crossing and Slit responsiveness of commissural axons require USP33. Nat Neurosci 12, 1087–1089 (2009). https://doi.org/10.1038/nn.2382
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DOI: https://doi.org/10.1038/nn.2382
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