The Sleeping Beauty (SB) transposon emerged as a useful tool for applications such as germline and somatic cell insertional mutagenesis and now shows its usefulness again by facilitating saturating germline mutagenesis in mice.
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Izsvák, Z., Ivics, Z. Sleeping Beauty hits them all: transposon-mediated saturation mutagenesis in the mouse germline. Nat Methods 2, 735–736 (2005). https://doi.org/10.1038/nmeth1005-735
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DOI: https://doi.org/10.1038/nmeth1005-735
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