Abstract
We describe Census, a quantitative software tool compatible with many labeling strategies as well as with label-free analyses, single-stage mass spectrometry (MS1) and tandem mass spectrometry (MS/MS) scans, and high- and low-resolution mass spectrometry data. Census uses robust algorithms to address poor-quality measurements and improve quantitative efficiency, and it can support several input file formats. We tested Census with stable-isotope labeling analyses as well as label-free analyses.
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References
Makarov, A. et al. Anal. Chem. 78, 2113–2120 (2006).
Olsen, J.V. et al. Mol. Cell. Proteomics 4, 2010–2021 (2005).
Yates, J.R., Cociorva, D., Liao, L. & Zabrouskov, V. Anal. Chem. 78, 493–500 (2006).
Denison, C. et al. Mol. Cell. Proteomics 4, 246–254 (2005).
Dieguez-Acuna, F.J. et al. Mol. Cell. Proteomics 4, 1459–1470 (2005).
Foster, L.J., Hoog, C.L.d. & Mann, M. Proc. Natl. Acad. Sci. 100, 5813–5818 (2003).
Venable, J.D., Wohlschlegel, J., McClatchy, D.B., Park, S.K. & Yates, J.R. III. Anal. Chem. 79, 3056–3064 (2007).
Eng, J.K., McCormack, A.L. & Yates, J.R., III . J. Am. Soc. Mass Spectrom. 5, 976–989 (1994).
Sadygov, R.G. & Yates, J.R., III . Anal. Chem. 75, 3792–3798 (2003).
Tabb, D.L., Saraf, A. & Yates, J.R., III . Anal. Chem. 75, 6415–6421 (2003).
Geer, L.Y. et al. J. Proteome Res. 3, 958–964 (2004).
Ong, S.E. & Mann, M. Nat. Chem. Biol. 1, 252–262 (2005).
Domon, B. & Aebersold, R. Science 312, 212–217 (2006).
MacCoss, M.J., Wu, C.C. & Yates, J.R., III . Anal. Chem. 75, 6912–6921 (2003).
Venable, J.D., Dong, M.Q., Wohlschlegel, J., Dillin, A. & Yates, J.R. Nat. Methods 1, 39–45 (2004).
Owens, K.G. Appl. Spectrosc. Rev. 27, 1–49 (1992).
Arnott, D. et al. Mol. Cell. Proteomics 1, 148–156 (2002).
Acknowledgements
We thank J. Wohlschlegel and D. Cociorva for their comments and discussions about the manuscript, M. MacCoss for his insights and work developing RelEx, S. Agarwalla and D. McMullan for sample preparation. S.K.P. is supported by US National Institutes of Health (NIH) National Institute of Allergy and Infectious Diseases (UOM/DMID-BAA-03). J.D.V. is supported by a National Research Service Award (NIH) fellowship. T.X. is supported by NIH (DE016267). J.R.Y. is supported by NIH (P41 RR011823, R01 MH067880 and R01 HL079442).
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Contributions
S.K.P. developed the algorithm and applications; J.D.V. prepared yeast cell lysates, 10 protein standard mixtures, iTRAQ samples and developed algorithms; T.X. contributed to discussions on algorithms; J.R.Y. led and coordinated the project.
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Supplementary information
Supplementary Text and Figures
Supplementary Figures 1–8, Supplementary Tables 1–6, Supplementary Methods (PDF 673 kb)
Supplementary Data
Quantitative analysis results obtained from Census for 1:1 mixture of unlabeled and metabolically 15N labeled yeast standards. (ZIP 145 kb)
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Park, S., Venable, J., Xu, T. et al. A quantitative analysis software tool for mass spectrometry–based proteomics. Nat Methods 5, 319–322 (2008). https://doi.org/10.1038/nmeth.1195
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DOI: https://doi.org/10.1038/nmeth.1195
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