Abstract
Diamond, because of its electrical and chemical properties, may be a suitable material for integrated sensing and signal processing. But methods to control chemical or biological modifications on diamond surfaces have not been established. Here, we show that nanocrystalline diamond thin-films covalently modified with DNA oligonucleotides provide an extremely stable, highly selective platform in subsequent surface hybridization processes. We used a photochemical modification scheme to chemically modify clean, H-terminated nanocrystalline diamond surfaces grown on silicon substrates, producing a homogeneous layer of amine groups that serve as sites for DNA attachment. After linking DNA to the amine groups, hybridization reactions with fluorescently tagged complementary and non-complementary oligonucleotides showed no detectable non-specific adsorption, with extremely good selectivity between matched and mismatched sequences. Comparison of DNA-modified ultra-nanocrystalline diamond films with other commonly used surfaces for biological modification, such as gold, silicon, glass and glassy carbon, showed that diamond is unique in its ability to achieve very high stability and sensitivity while also being compatible with microelectronics processing technologies. These results suggest that diamond thin-films may be a nearly ideal substrate for integration of microelectronics with biological modification and sensing.
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References
Buriak, J.M. Organometallic chemistry on silicon and germanium surfaces. Chem. Rev. 102, 1271–1308 (2002).
Wei, J., Smentkowski, V.S. & Yates, J.T. Jr. Diamond surface chemistry. 2. Selected bibliography. Crit. Rev. Surf. Chem. 5, 73–248 (1995).
Bousse, L., de Rooij, N.F. & Bergveld, P. Operation of chemically sensitive field-effect sensors as a function of insulator-electrolyte interface. IEEE Trans. Electron Dev. 30, 1263–1270 (1983).
Linford, M.R., Fenter, P., Eisenberger, P.M. & Chidsey, C.E.D. Alkyl monolayers on silicon prepared from 1-alkenes and hydrogen-terminated silicon. J. Am. Chem. Soc. 117, 3145–3155 (1995).
Strother, T. et al. Photochemical functionalization of diamond films. Langmuir 18, 968–971 (2002).
Strother, T., Cai, W., Zhao, X., Hamers, R.J. & Smith, L.M. Synthesis and characterization of DNA-modified silicon (111) surfaces. J. Am. Chem. Soc. 122, 1205–1209 (2000).
Strother, T., Hamers, R.J. & Smith, L.M. Covalent attachment of oligodeoxyribonucleotides to amine-modified Si (001) surfaces. Nucleic Acids Res. 28, 3535–3541 (2000).
Mathieu, H.J. Bioengineered material surfaces for medical applications. Surf. Interface Anal. 32, 3–9 (2001).
Granger, M.C. et al. Standard electrochemical behavior of high-quality, boron-doped polycrystalline diamond thin-film electrodes. Anal. Chem. 72, 3793–3804 (2000).
Swain, G.M. & Ramesham, M. The electrochemical activity of boron-doped polycrystalline diamond thin-film electrodes. Anal. Chem. 65, 345–351 (1993).
Cui, F.Z. & Li, D.J. A review of investigations on biocompatibility of diamond-like carbon and carbon nitride films. Surf. Coat. Technol. 131, 481–487 (2000).
Tang, L., Tasi, C., Gerberich, W.W., Kruckeberg, L. & Kanie, D.R. Biocompatibilitiy of chemical-vapor-deposited diamond. Biomaterials 16, 483–488 (1995).
Rotter, S. Applications of conformal CVD diamond films. Israel J. Chem. 38, 135–140 (1998).
May, P.W. Diamond thin films: a 21st-century material. Phil. Trans. R. Soc. Lond. A 358, 473–495 (2000).
Gruen, D.M. Nanocrystalline diamond films. Ann. Rev. Mater. Sci. 29, 211–259 (1999).
Butler, J.E. & Windischmann, H. Developments in CVD-diamond synthesis during the past decade. Mater. Res. Bull. 23, 22–27 (1998).
Corrigan, T.D., Krauss, A.R., Gruen, D.M., Auciello, O. & Chang, R.P.H. Low temperature growth of ultrananocrystalline diamond on glass substrates for field emission applications. Mater. Res. Soc. Symp. Proc. 593, 233–236 (2000).
Liu, H.M. & Dandy, D.S. Studies on nucleation process in diamond CVD - an overview of recent developments. Diam. Relat. Mater. 4, 1173–1188 (1995).
Thoms, B.D., Owens, M.S., Butler, J.E. & Spiro, C. Production and characterization of smooth, hydrogen-terminated diamond C(100). Appl. Phys. Let. 65, 2957–2959 (1994).
Thoms, B.D. & Butler, J.E. HREELS and LEED of H/C(100): The 2× 1 monohydride dimer row reconstruction. Surf. Sci. 328, 291–301 (1995).
Rahn, R.O. Potassium iodide as a chemical actinometer for 254 nm radiation: Use of iodate as an electron scavenger. Photochem. Photobiol. 66, 450–455 (1997).
Wilks, J. & Wilks, E. Properties and Applications of Diamond (Butterworth-Heinemann, Oxford, 1991).
Cai, W., Lin, Z., Strother, T., Smith, L.M. & Hamers, R.J. Chemical modification and patterning of iodine-terminated silicon surfaces using visible light. J. Phys. Chem. B. 106, 2656–2664 (2002).
King, S.B. & Ganem, B. Synthetic studies on Mannostatin A and its derivatives: a new family of glycoprotein processing inhibitors. J. Am. Chem. Soc. 116, 562–570 (1994).
Frutos, A.G., Smith, L.M. & Corn, R.M. Enzymatic ligation reactions of DNA “words” on surfaces for DNA computing. J. Am. Chem. Soc. 120, 10277–10282 (1998).
Lin, Z. et al. DNA attachment and hybridization at the silicon (100) surface. Langmuir 18, 788–796 (2002).
Everett, W.R. & Fritschfaules, I. Factors that influence the stability of self-assembled organothiols on gold under electrochemical conditions. Anal. Chim. Acta 307, 253–268 (1995).
Gray, D.E., Case-Green, S.C., Fell, T.S., Dobson, P.J. & Southern, E.M. Ellipsometric and interferometric characterization of DNA probes immobilized on a combinatorial array. Langmuir 13, 2833–2842 (1997).
Major, R.C. & Zhu, X.-Y. Two-step approach to the formation of organic monolayers on the silicon oxide surface. Langmuir 17, 5576–5580 (2001).
Finne, R.M. & Klein, D.L. A water-amine complexing agent system for etching silicon, J. Electrochem. Soc. 114, 965–970 (1967).
Acknowledgements
The authors acknowledge the assistance of Thomas Beebe and Matthew Wells. This work was supported in part by the US Office of Naval Research N00014-01-1-0654, the Wisconsin Alumni Research Foundation, the National Institutes of Health Grant R01 EB00269, the National Science Foundation and the US Department of Energy, BES-Materials Sciences, under Contract W-31-109-ENG-38.
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Yang, W., Auciello, O., Butler, J. et al. DNA-modified nanocrystalline diamond thin-films as stable, biologically active substrates. Nature Mater 1, 253–257 (2002). https://doi.org/10.1038/nmat779
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DOI: https://doi.org/10.1038/nmat779
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