Abstract
Traditional methods for fabricating nanoscale arrays are usually based on lithographic techniques. Alternative new approaches rely on the use of nanoscale templates made of synthetic or biological materials. Some proteins, for example, have been used to form ordered two-dimensional arrays. Here, we fabricated nanoscale ordered arrays of metal and semiconductor quantum dots by binding preformed nanoparticles onto crystalline protein templates made from genetically engineered hollow double-ring structures called chaperonins. Using structural information as a guide, a thermostable recombinant chaperonin subunit was modified to assemble into chaperonins with either 3 nm or 9 nm apical pores surrounded by chemically reactive thiols. These engineered chaperonins were crystallized into two-dimensional templates up to 20 μm in diameter. The periodic solvent-exposed thiols within these crystalline templates were used to size-selectively bind and organize either gold (1.4, 5 or 10nm) or CdSe–ZnS semiconductor (4.5 nm) quantum dots into arrays. The order within the arrays was defined by the lattice of the underlying protein crystal. By combining the self-assembling properties of chaperonins with mutations guided by structural modelling, we demonstrate that quantum dots can be manipulated using modified chaperonins and organized into arrays for use in next-generation electronic and photonic devices.
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Acknowledgements
The authors thank A. P. Alivisatos and W. J. Parak for the semiconductor quantum dots, M. Wilson and Y. F. Li for assistance in assembling chaperonin models, A. Madhukar for quantum dot discussions, and R. Boyle and J. Varelas of the NASA BioVis Center for use of the LEO TEM. This work was supported through funding from the NASA Ames Center for Nanotechnology. We also acknowledge funding from the US Department of Energy, the Defense Advanced Research Projects Agency, and the NASA Ames Director's Discretionary Fund. Work at ANL was supported in part by the US DoE BES-MS W-31-109-Eng-38.
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McMillan, R., Paavola, C., Howard, J. et al. Ordered nanoparticle arrays formed on engineered chaperonin protein templates. Nature Mater 1, 247–252 (2002). https://doi.org/10.1038/nmat775
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DOI: https://doi.org/10.1038/nmat775
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