Restoration of genes lost during the original attenuation of the commonly used BCG tuberculosis vaccine enhances the ability of a recombinant strain to protect against Mycobacterium tuberculosis.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Pym, A.S. et al. Recombinant BCG exporting ESAT-6 confers enhanced protection against tuberculosis. Nat. Med. 9; advance online publication 7 April 2003; doi:10.1038/nm859.
Lewis, K.N. et al. Deletion of RD1 from Mycobacterium tuberculosis mimics Bacille Calmette-Guérin attenuation. J. Infect. Dis. 183, 117–123 (2003).
Pym, A.S., Brodin, P., Brosch, R., Huerre, M. & Cole, S.T. Loss of RD1 contributed to the attenuation of the live tuberculosis vaccines Mycobacterium bovis BCG and Mycobacterium microti. Mol. Microbiol. 46, 709–712 (2002).
Chapman, A.L. et al. Rapid detection of active and latent tuberculosis infection in HIV-positive individuals by enumeration of Mycobacterium tuberculosis-specific T cells. AIDS 16, 2285–2293 (2002).
Brandt, L., Elhay, M., Rosenkrands, I., Lindblad, E.B. & Andersen, P. ESAT-6 subunit vaccination against Mycobacterium tuberculosis. Infect. Immun. 68, 791–795 (2000).
Renshaw, P.S. et al. Conclusive evidence that the major T-cell antigens of the M. tuberculosis complex form a tight 1:1 complex and characterisation of the structural properties of ESAT-6, CFP-10 and the ESAT-6-CFP-10 complex: implications for pathogenesis and virulence. J. Biol. Chem. 277, 21598–21603 (2002).
Horwitz, M.A., Harth, G., Dillon, B.J. & Maslesa-Galic, S. Recombinant Bacillus Calmette-Guérin (BCG) vaccines expressing the Mycobacterium tuberculosis 30-kDa major secretory protein induce greater protective immunity against tuberculosis than conventional BCG vaccines in a highly susceptible animal model. Proc. Natl. Acad. Sci. USA 97, 13853–13858 (2000).
Cole, S.T. et al. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393, 537–544 (1998).
Gey Van Pittius, N.C. et al. The ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteria. Genome Biol. 2, 1–18 (2001).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Young, D. Building a better tuberculosis vaccine. Nat Med 9, 503–504 (2003). https://doi.org/10.1038/nm868
Published:
Issue Date:
DOI: https://doi.org/10.1038/nm868
This article is cited by
-
Recommendations for the diagnosis of pediatric tuberculosis
European Journal of Clinical Microbiology & Infectious Diseases (2016)