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Identification of endothelial cell–surface proteins as targets for diagnosis and treatment of disease

Nature Medicine volume 2, pages 482–484 (1996)Cite this article

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References

  1. Folkman, J. Tumor angiogenesis: Therapeutic implications. N. Engl. J. Med. 285, 1182–1186 (1971).

    Article  CAS  Google Scholar 

  2. Burrows, F.J. & Thorpe, P.E. Eradication of large solid tumors in mice with an immunotoxin directed against tumor vasculature. Proc. Natl. Acad. Sci. USA 90, 8996–9000 (1993).

    Article  CAS  Google Scholar 

  3. Brooks, P.C., Clark, R.A.F. & Cheresh, D.A. Requirement of vascular integrin αvβ3 for angiogenesis. 264, 569–571 (1994).

  4. Buck, C.A. Adhesion mechanism controlling cell-cell and cell-matrix interactions during the metastatic process. in The Molecular Basis of Cancer. (eds. Mendelsohn, J. et al.) 172–205 (Saunders, Philadelphia, 1995).

    Google Scholar 

  5. Schwartz, L.M., Milligan, C.E., Bielke, W. & Robinson, S.J. Cloning cell death genes. Methods Cell Biol. 46, 107–138 (1995).

    Article  CAS  Google Scholar 

  6. Jacobson, B.S. & Branton, D. Plasma membranes: Rapid isolation and exposure of the cytoplasmic surface by use of positively charged beads. Science 195, 302–304 (1977).

    Article  CAS  Google Scholar 

  7. Chaney, L.K. & Jacobson, B.S. Coating cells with colloidal silica for high yield isolation of plasma membrane sheets and identification of transmembrane proteins. J. Biol. Chem. 258, 10062–10072 (1983).

    CAS  PubMed  Google Scholar 

  8. Stolz, D.B., Branton, D. & Jacobson, B.S. Plasma membrane isolation using the cationic colloidal silica isolation technique. in Cell Biology: A Laboratory Manual. (Cold Spring Harbor Press, Cold Spring Harbor, New York, in the press).

  9. Jacobson, B.S., Schnitzer, J.E., McCaffery & Palade, G.E. Isolation and partial characterization of the luminal plasmalemma of microvascular endothelium from rat lungs. Eur. J. Cell Biol. 58, 296–306 (1992).

    CAS  PubMed  Google Scholar 

  10. Stolz, D.B., Bannish, G. & Jacobson, B.S. The role of the cytoskeleton and intercellular junctions on the transcellular membrane protein polarity of bovine aortic endothelial cells in vitro. J. Cell Sci. 103, 53–68 (1992).

    CAS  PubMed  Google Scholar 

  11. Shiozawa, J.A., Jelenska, M.M. & Jacobson, B.S. Topography of the Dictyostelium discoideum plasma membrane: Analysis of membrane asymmetry and intermolecular disulfide bonds. Biochemistry 26, 4884–4892 (1987).

    Article  CAS  Google Scholar 

  12. Patton, W.F., Dhanak, M.R. & Jacobson, B.S. Identification of Dictyostelium discoideum plasma membrane proteins by cell surface labeling and quantitative two-dimensional gel electrophoresis. Anal. Biochem. 179, 37–49 (1989).

    Article  CAS  Google Scholar 

  13. Patton, W.F., Dhanak, M.R. & Jacobson, B.S. Differential partitioning of plasma membrane proteins into the Triton-X-100 insoluble cytoskeleton of Dictyostelium discoideum. J. Cell Sci. 92, 85–91 (1989).

  14. Schnitzer, J.E., Oh, P., Jacobson, B.S. & Dvorak, A.M. Caveolae from luminal plasmalemma of rat lung endothelium: Microdomains enriched in caveolin, Ca2+-ATPase, and inositol triphosphate receptor. Proc. Natl. Acad. Sci. USA 92, 1759–1763 (1995).

    Article  CAS  Google Scholar 

  15. Schnitzer, J.E., Mclntosh, D.P., Dvorak, A.M., Liu, J. & Oh, P. Separation of caveolae from associated microdomains of GPI-anchored proteins. Science 269, 1435–1439 (1995).

    Article  CAS  Google Scholar 

  16. Schnitzer, J.E. Update of the cellular and molecular basis of capillary permeability. Trends Cardiovasc. Med. 3, 124–130 (1993).

    Article  CAS  Google Scholar 

  17. Stolz, D.B. & Jacobson, B.S. Macro- and micro vascular endothelial cells in vitro: Maintenance of biochemical heterogeneity despite loss of ultrastructural characteristics. In Vitro Cell Dev. Biol. 27A, 169–182 (1991).

    Article  CAS  Google Scholar 

  18. Blood, C.H. & Zetter, B.R. Tumor interactions with the vasculature: angiogenesis and tumor metastasis. Biochim. Biophys. Ada 1032, 89–118 (1990)

    CAS  Google Scholar 

  19. Folkman, J. Clinical applications of research on angiogenesis. N. Engl. J. Med. 33, 1757–1763 (1995).

    Article  Google Scholar 

  20. Gerritsen, M.E. & Bloor, C.M. Endothelial cell gene expression in response to injury. FASEB J. 7, 523–532 (1993).

    Article  CAS  Google Scholar 

  21. Bauw, G. et al. Two-dimensional gel electrophoresis, protein electroblotting and microsequencing: A direct link between proteins and genes. Electrophoresis 11, 528–536 (1990).

    Article  CAS  Google Scholar 

  22. Bieman, K. Mass spectrometry of peptides and proteins. Annu. Rev. Biochem. 61, 977–1010 (1992).

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Massachusetts, 01003, USA

    Bruce S. Jacobson

  2. Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, 15261, USA

    Donna B. Stolz

  3. Department of Pathology, Harvard Medical School, Beth Israel Hospital, 330 Brookline Avenue, Boston, Massachusetts, 02215, USA

    Jan E. Schnitzer

Authors
  1. Bruce S. Jacobson
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  2. Donna B. Stolz
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  3. Jan E. Schnitzer
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Correspondence to Bruce S. Jacobson.

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Jacobson, B., Stolz, D. & Schnitzer, J. Identification of endothelial cell–surface proteins as targets for diagnosis and treatment of disease. Nat Med 2, 482–484 (1996). https://doi.org/10.1038/nm0496-482

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