If doing cutting-edge research is like running a marathon, then in the field of molecular biology, DNA cloning — inserting known sequences of DNA into an organism to amplify them — is the equivalent of tying your shoelaces. A basic skill that you learn as an undergraduate, DNA cloning is usually the starting point for getting more complex experiments up and running. This procedure is a familiar routine. So when I recently encountered a problem it was like falling over my own two feet. I was surprised and embarrassed.

Getting my momentum back was a process. First there was denial: “This can't be happening.” Then came anger: “*$#!#*&%! protocol!” Bargaining and abject begging followed: “Please, just let this experiment work...” Next came depression: “I'm never going to finish this project — my career is over.” And, finally, acceptance settled in: “It's time to re-evaluate my strategy and to move on.”

I had to get back on track after being thrown off course by this unexpected obstacle. Despite a slightly bruised ego, I picked myself up, dusted myself down and kept going, and my resilience paid off. Relief and redemption came in the form of small bacterial colonies. Back on my feet, I'm up and running once again.