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Telomere–associated chromosome fragmentation: applications in genome manipulation and analysis

Nature Genetics volume 2, pages 275–282 (1992)Cite this article

Abstract

Telomere–associated chromosome fragmentation (TACF) is a new approach for chromosome mapping based on the non–targeted introduction of cloned telomeres into mammalian cells. TACF has been used to generate a panel of somatic cell hybrids with nested terminal deletions of the long arm of the human X chromosome, extending from Xq26 to the centromere. This panel has been characterized using a series of X chromosome loci. Recovery of the end clones by plasmid rescue produces a telomeric marker for each cell line and partial sequencing will allow the generation of sequence tagged sites (STSs). TACF provides a powerful and widely applicable method for genome analysis, a general way of manipulating mammalian chromosomes and a first step towards constructing artificial mammalian chromosomes.

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Author notes

  1. Christine J. Farr, Milena Stevanovic & Peter N. Goodfellow

    Present address: Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK

Authors and Affiliations

  1. Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London, WC2A 3PX, UK

    Christine J. Farr, Milena Stevanovic & Peter N. Goodfellow

  2. Institute of Molecular Genetics & Genetic Engineering, Vojvode Stepe 283, 11001, Belgrade, Yugoslavia

    Milena Stevanovic

  3. MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh, EX4 2XU, UK

    Eric J. Thomson & Howard J. Cooke

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  5. Howard J. Cooke
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Farr, C., Stevanovic, M., Thomson, E. et al. Telomere–associated chromosome fragmentation: applications in genome manipulation and analysis. Nat Genet 2, 275–282 (1992). https://doi.org/10.1038/ng1292-275

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