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Telomeric localization of TRF2, a novel human telobox protein

Nature Genetics volume 17, pages 236–239 (1997)Cite this article

Abstract

Natural chromosomal ends are stabilized by proteins that bind duplex telomeric DNA repeats. In human cells, the TTAGGG Repeat Factor 1 (TRF1) was identified by two independent studies, one screening for factors that bind duplex telomeric DNA1,2 and the other screening for proteins containing a particular Myb motif called the telobox, which is required for telomeric repeat recognition (Fig. 1a; refs 3–5). A second human open reading frame, orf2, contains a telobox sequence and encodes a polypeptide that specifically recognizes mammalian telomeric repeat DNA in vitro3. We show that two proteins of 65 and 69 kD, expressed in HeLa cells, contain the orf2 telobox sequence. These proteins are collectively termed TRF2. Affinity-purified antibodies specific for anti-TRF2 label the telomeres of intact human chromosomes, strengthening the correlation between occurrence of telobox and telomere-repeat recognition in vivo.

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Author notes

  1. Thomas Bilaud and Christine Brun: T.B. and C.B. contributed equally to this work.

Authors and Affiliations

  1. Laboratoire de Biologic Moléculaire et Cellulaire, UMR49, Centre National de la Recherche Scientifique, Ecole Normale Supériure de Lyon, 46 Allée d'Italie, 69364, Lyon, Cedex 07, France

    Thomas Bilaud, Christine Brun, Katia Ancelin, Catherine Elaine Koering & Eric Gilson

  2. Swiss Institute for Experimental Cancer Research (ISREC), Chemin des Boveresses 155, CH-1066, Epalinges/Lausanne, Switzerland

    Thierry Laroche

Authors
  1. Thomas Bilaud
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  2. Christine Brun
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  3. Katia Ancelin
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  4. Catherine Elaine Koering
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  5. Thierry Laroche
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  6. Eric Gilson
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Correspondence to Eric Gilson.

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Bilaud, T., Brun, C., Ancelin, K. et al. Telomeric localization of TRF2, a novel human telobox protein. Nat Genet 17, 236–239 (1997). https://doi.org/10.1038/ng1097-236

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