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Construction of a mouse yeast artificial chromosome library in a recombination–deficient strain of yeast

Nature Genetics volume 1, pages 132–136 (1992)Cite this article

Abstract

We have constructed a new generation yeast artificial chromosome (YAC) library from female C57BL/10 mice in a recombination–deficient strain of Saccharomyces cerevisiae carrying a mutation in the RAD52 gene. The YAC library contains 41,568 clones with an average insert size of 240 kilobases, representing a greater than threefold coverage of the mouse genome. Currently, the library can be screened by polymerase chain reaction and we have isolated positive clones at a number of loci in the mouse genome. This rad52 library should enable a long–term assessment of the effect of one of the yeast recombination pathway genes on both, genome–wide YAC clone stability and the frequency of chimaeric clones.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, St Mary's Hospital Medical School, Norfolk Place, London, W2 1PG, UK

    F. L. Chartier, J. T. Keer, M. J. Sutcliffe, D. A. Henriques, P. Mileham & S. D. M. Brown

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  1. F. L. Chartier
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  2. J. T. Keer
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  3. M. J. Sutcliffe
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  4. D. A. Henriques
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  6. S. D. M. Brown
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Chartier, F., Keer, J., Sutcliffe, M. et al. Construction of a mouse yeast artificial chromosome library in a recombination–deficient strain of yeast. Nat Genet 1, 132–136 (1992). https://doi.org/10.1038/ng0592-132

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