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Transplantation to the rat brain of human neural progenitors that were genetically modified using adenoviruses

Nature Genetics volume 9, pages 256–260 (1995)Cite this article

Abstract

Transplantations for neurological disorders are limited by the supply of human fetal tissue. To generate larger numbers of cells of appropriate phenotype, we investigated whether human neural progenitors expanded in vitro could be modified with recombinant adenoviruses. Strong expression of β–galactosidase was obtained in vitro. Two or three weeks after transplantation of engineered cells to the rat brain, we observed a small percentage of surviving neuroblasts strongly expressing β–galactosidase in four out of 13 rats. Thus human precursor cells that have been genetically modified using adenoviruses are a promising tool for ex vivo gene therapy of neurodegenerative diseases.

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Authors and Affiliations

  1. C 9923 CNRS, Laboratoire de Génétique Moléculaire de la Neurotransmission et des Processus Neurodégénératifs, 1 Avenue de la Tetrasse, 91198, Gifsur-Yvette, France

    Olivier Sabaté, Philippe Horellou, Philippe Colin & Jacques Mallet

  2. URA 1301 CNRS, Institut Gustave Roussy, PR2, 39 rue Camille Desmoulins, 94805, Villejuif, France

    Emmanuelle Vigne & Michel Perricaudet

  3. Département de Biologie Moléculaire et ERS 67 du CNRS, Institut Pasteur, 25 rue du Docteur Roux, 75274, Paris Cedex, 15, France

    Marie-Hélène Buc-Caron

Authors
  1. Olivier Sabaté
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  2. Philippe Horellou
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  3. Emmanuelle Vigne
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  4. Philippe Colin
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  6. Marie-Hélène Buc-Caron
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  7. Jacques Mallet
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Sabaté, O., Horellou, P., Vigne, E. et al. Transplantation to the rat brain of human neural progenitors that were genetically modified using adenoviruses. Nat Genet 9, 256–260 (1995). https://doi.org/10.1038/ng0395-256

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