Abstract
IT is now generally accepted that the release of calcium ions from intracellular stores (presumably the sarcoplasmic reticulum (SR)) is a key step in excitation-contraction coupling in skeletal muscle1–4. One of the most useful preparations for studying the mechanism of release is the mechanically skinned muscle fibre preparation, originally devised by Natori5, and subsequently modified for use in aqueous solutions 6–9. The skinned fibre has the advantage that the cell membrane is removed, allowing free access of substances placed in the bathing medium to intracellular structures, but at the same time causing minimal disruption of these structures. The partially skinned (split) fibre has the further advantage that the patency of the T tubules is maintained13.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
We are sorry, but there is no personal subscription option available for your country.
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Sandow, A., Pharmac. Rev., 17, 265 (1965).
Weber, A., Curr. Top. Bioenergetics, 1, 203 (1966).
Ebashi, S., and Endo, M., Prog. Biophys. molec. Biol., 18, 123 (1968).
Ebashi, S., Endo, M., and Ohtsuki, I., Q. Rev. Biophys., 2, 351 (1969).
Natori, R., Jikeikai med. J., 1, 119 (1954).
Hellam, D. C., and Podolsky, R. J., J. Physiol. Lond., 200, 807 (1969).
Endo, M., Proc. Seventeenth gen. Assoc. Japan. med. Congr., 1, 193 (1967).
Reuben, J. P., Brandt, P. W., Berman, M., and Grundfest, H., J. gen. Physiol., 57, 385 (1971).
Gordon, A. M., Godt, R. E., and Woodbury, J. W., Fed. Proc., 29, 656 (1970).
Ford, L. E., and Podolsky, R. J., Science, N.Y., 167, 58 (1970).
Endo, M., Tanaka, M., and Ogawa, Y., Nature, 228, 34 (1970).
Ford, L. E., and Podolsky, R. J., J. Physiol. Lond., 223, 1 (1972).
Nakajima, Y., and Endo, M., Nature new Biol., 246, 216 (1973).
Ford, L. E., and Podolsky, R. J., J. Physiol. Lond., 223, 21 (1972).
Shimomura, O., Johnson, F. H., and Saiga, Y., J. cell. comp. Physiol., 59, 223 (1962).
Ashley, C. C., and Ridgway, E. B., J. Physiol. Lond., 209, 105 (1970).
Blinks, J. R., Proc. Int. Union Physiol. Sci., 9, 68 (1971).
Blinks, J. R., Mattingly, P. H., Jewell, B. R., and van Leeuwen, M., Fed. Proc., 28, 781 (1969).
Ogawa, Y., J. Biochem., 64, 255 (1968).
Orentlicher, M., Reuben, J. P., and Brandt, P. W., Biophys. Soc., abstr. 12, 81a (1972).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
ENDO, M., BLINKS, J. Inconstant Association of Aequorin Luminescence with Tension during Calcium Release in Skinned Muscle Fibres. Nature New Biology 246, 218–221 (1973). https://doi.org/10.1038/newbio246218a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/newbio246218a0
This article is cited by
-
The effects of quinine on the calcium and magnesium content of the sarcoplasmic reticulum and the temperature-dependence of quinine contractures
Journal of Muscle Research and Cell Motility (1987)
-
Calcium and magnesium binding to thin and thick filaments in skinned muscle fibres: electron probe analysis
Journal of Muscle Research and Cell Motility (1982)
-
Chloride-induced release of actively loaded calcium from light and heavy sarcoplasmic reticulum vesicles
The Journal of Membrane Biology (1980)