Abstract
L CELLS may be grown in defined medium either in monolayer culture or as suspension cultures in spinner flasks. The adaptation of these cells from one growth behaviour to the other, however, requires the time consuming selection of proper mutants and/or changes in the composition of their medium (Ca2+, Mg2+ and serum concentration) probably causing further selection among the population.
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References
Bremerskov, V., and Linnemann, R., Eur. J. Cancer, 5, 317 (1969).
Terasima, T., and Tolmach, L. J., Expl Cell Res., 30, 344 (1963).
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BREMERSKOV, V. Dextran Sulphate inhibits Cell Adhesion in Tissue Culture. Nature New Biology 246, 174 (1973). https://doi.org/10.1038/newbio246174a0
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DOI: https://doi.org/10.1038/newbio246174a0