Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letters to Editor
  • Published:

Two Proteins of Gene A of ϕX174

Nature New Biology volume 245, pages 6–8 (1973)Cite this article

Abstract

GENE A of the single strand DNA phages ϕX174 and S13 is the only phage gene required for the replication of the double stranded replicative form (RF) after the complementary strand of DNA is synthesized upon the infecting single strand DNA1–3. It is a cis acting gene, double infection with gene A mutants and mutants in the other phage genes fail to rescue mutant A phage4, suggesting that gene A products can only function upon the genome from which they are synthesized. Gene A is necessary for the detection of a cis related viral strand specific discontinuity in the parental RF5. The gene A region of the ϕX174 genome has been suggested to contain the origin of DNA replication6 and genetic evidence has been presented to support the model that gene A produces two proteins, A and A′ (molecular weight 35,000 and 60–65,000, respectively)7.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

References

  1. Tessman, E. S., J. molec. Biol., 17, 218 (1966).

    Article  CAS  Google Scholar 

  2. Lindqvist, B. H., and Sinsheimer, R. L., J. molec, Biol., 28, 87 (1967).

    Article  CAS  Google Scholar 

  3. Iwaya, M., and Denhardt, D. T., J. molec. Biol., 57, 159 (1971).

    Article  CAS  Google Scholar 

  4. Tessman, E. S., Virology, 25, 303 (1965).

    Article  CAS  Google Scholar 

  5. Francke, B., and Ray, D. S., Proc. natn. Acad. Sci. U.S.A., 69, 475 (1972).

    Article  CAS  Google Scholar 

  6. Baas, P. D., and Jansz, H. S., J. molec. Biol., 63, 569 (1972).

    Article  CAS  Google Scholar 

  7. Linney, E. A., Hayashi, M. N., and Hayashi, M., Virology, 50, 381 (1972).

    Article  CAS  Google Scholar 

  8. Weiner, A. M., and Weber, E., Nature new Biol., 234, 206 (1971).

    Article  CAS  Google Scholar 

  9. Vanderbilt, A. S., and Tessman, I., Nature, 228, 54 (1970).

    Article  CAS  Google Scholar 

  10. Lenard, J., Biochem. biophys. Res. Commun., 45, 662 (1971).

    Article  CAS  Google Scholar 

  11. Gelfand, D. H., and Hayashi, M., Proc. natn. Acad. Sci. U.S.A., 67, 13 (1970).

    Article  CAS  Google Scholar 

  12. Hayashi, M., Hayashi, M. N., and Spiegelman, S., Proc. natn. Acad. Sci. U.S.A., 50, 664 (1963).

    Article  CAS  Google Scholar 

  13. Tessman, I., Komer, S., and Tessman, E. S., Science, 158, 267 (1967).

    Article  CAS  Google Scholar 

  14. Vanderbilt, A. S., Borras, M., and Tessman, E. S., Virology, 48, 352 (1971).

    Article  Google Scholar 

  15. Gelfand, D. H., and Hayashi, M., J. molec. Biol., 44, 501 (1969).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, University of California, San Diego, La Jolla, California, 92037

    ELWOOD LINNEY & MASAKI HAYASHI

Authors
  1. ELWOOD LINNEY
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. MASAKI HAYASHI
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

LINNEY, E., HAYASHI, M. Two Proteins of Gene A of ϕX174. Nature New Biology 245, 6–8 (1973). https://doi.org/10.1038/newbio245006a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/newbio245006a0

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing