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Production of Possible Recombination Intermediates by an ATP-dependent DNAase

Nature New Biology volume 241, pages 54–58 (1973)Cite this article

Abstract

ATP-DEPENDENT DNAases have been found in several bacteria (Escherichia coli1–4, Micrococcus lysodeikticus5, Diplococcus pneumoniae6, Haemophilus influenzae7–11, Mycobacterium smegmatis12 and Bacillus laterosporus13) and are thought to be “recombination enzymes”. This is based on the observations that recombination deficient recB and C mutants of E. coli14,15 are deficient in an ATP-dependent DNAase activity and that D. pneumoniae mutants deficient in this enzyme show decreased levels of genetic transformation16. The DNAases from E. coli2–4 and H. influenzae7–11 have in common the ability to degrade double-stranded DNA exonucleolytically and single-stranded DNA endonucleolytically, while at the same time hydrolysing large amounts of ATP to ADP.

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Authors and Affiliations

  1. Department of Microbiology, Johns Hopkins University, School of Medicine, Baltimore, Maryland, 21205

    EILEEN A. FRIEDMAN & H. O. SMITH

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  1. EILEEN A. FRIEDMAN
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  2. H. O. SMITH
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FRIEDMAN, E., SMITH, H. Production of Possible Recombination Intermediates by an ATP-dependent DNAase. Nature New Biology 241, 54–58 (1973). https://doi.org/10.1038/newbio241054a0

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