Abstract
3′ 5′-CYCLIC adenosine monophosphate (cAMP) has been reported to be involved in the regulation of cellular growth1–5. cAMP levels are low in growing 3T3 cells whereas they are increased in resting cells in confluent cultures5. SV40 or polyoma virus transformed 3T3 cells as well as 3T6 and BHK cells, which reach high cell densities and do not become quiescent, are characterized by a low serum requirement and low levels of cAMP6 (W. Seifert, unpublished results). Therefore, it has been suggested that the growth pattern of normal cells (for example, density dependent cessation of growth) is controlled by intracellular cAMP levels2,5,6: high cellular levels of cAMP are considered to be growth inhibitory while depressed cellular cAMP levels may lead to a continuation of growth1,2,5. It has been suggested1,8 that the levels of cAMP in resting cells are increased as a consequence of contact between cells which may lead to a stimulation of the membrane bound adenyl cyclase. A negative signal which would lead to the inhibition of DNA synthesis as a consequence of cell-cell contact has been suggested7. Evidence from studies on inhibition of cell growth by (But)2cAMP and/or theophylline points to cAMP as a possible mediator1,2,8. Serum, however, antagonizes the growth inhibiting effect of (But)2cAMP9,10.
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SEIFERT, W., PAUL, D. Levels of Cyclic AMP in Sparse and Dense Cultures of Growing and Quiescent 3T3 Cells. Nature New Biology 240, 281–283 (1972). https://doi.org/10.1038/newbio240281a0
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DOI: https://doi.org/10.1038/newbio240281a0
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