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Intermediate in SV40 DNA Chain Growth

Nature New Biology volume 238, pages 274–277 (1972)Cite this article

Abstract

PREVIOUS studies of the DNA replication of simian virus 40 (SV40), an oncogenic member of the papoyavirus group, have been concerned with separation and characterization of replicative intermediates1–4. Circular replicating intermediates have been identified for SV401–3, as well as for the similar replication system of polyoma viral DNA5,6. The replicative intermediates of SV40 DNA have been observed by electron microscopy to contain two forks, three branches and no free ends1–3 as is the case for the circular replicating molecules of polyoma, bacteriophage λ7, Escherichia coli8, and colicin E1 in mini-cells9,10. An important property of replicative intermediates of SV40 DNA that has also been observed in replicating molecules of colicin E110 is that most molecules contain a superhelical region in the unreplicated portion of the molecule1.

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References

  1. Sebring, E. D., Kelly, jun., T. J., Thoren, M. M., and Salzman, N. P., J. Virol., 8, 478 (1971).

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Levine, A. J., Kang, H. S., and Billheimer, F. W., J. Mol. Biol., 50, 549 (1970).

    Article  CAS  Google Scholar 

  3. Jaenisch, R. J., Mayer, A., and Levine, A., Nature New Biology, 233, 72 (1971).

    Article  CAS  Google Scholar 

  4. Fareed, G. C., and Salzman, N. P., Fed. Proc., 31, 442, Abst. (1972).

    Google Scholar 

  5. Hirt, B., J. Mol. Biol., 40, 141 (1969).

    Article  CAS  Google Scholar 

  6. Bourgaux, P., Bourgaux-Ramoisy, D., and Dulbecco, R., Proc. US Nat. Acad. Sci., 64, 701 (1969).

    Article  CAS  Google Scholar 

  7. Ogawa, T., Tomizawa, J., and Fuke, M., Proc. US Nat. Acad. Sci., 60, 861 (1968).

    Article  CAS  Google Scholar 

  8. Cairns, J., Cold Spring Harbor Symp. Quant. Biol., 28, 43 (1963).

    Article  Google Scholar 

  9. Inselburg, J., and Fuke, M., Proc. US Nat. Acad. Sci., 68, 2839 (1971).

    Article  CAS  Google Scholar 

  10. Fuke, M., and Inselburg, J., Proc. US Nat. Acad. Sci., 69, 89 (1972).

    Article  CAS  Google Scholar 

  11. Gilbert, W., and Dressier, Cold Spring Harbor Symp. Quant. Biol., 33, 473 (1968).

    Article  CAS  Google Scholar 

  12. Okazaki, R., Okazaki, T., Sakabe, K., Sugimoto, K., and Sugino, A., Proc. US Nat. Acad. Sci., 59, 598 (1968).

    Article  CAS  Google Scholar 

  13. Okazaki, R., Okazaki, T. O., Sakabe, K., Sugimoto, K., Kainuma, R., Sugino, A., and Iwatsuki, N., Cold Spring Harbor Symp. Quant. Biol., 33, 129 (1968).

    Article  Google Scholar 

  14. Oishi, M., Proc. US Nat. Acad. Sci., 60, 329 (1968).

    Article  CAS  Google Scholar 

  15. Wang, H. F., and Sternglanz, R., Fed. Proc., 31, 441, Abstr. (1972).

    Google Scholar 

  16. Crawford, L. A., and Black, P. H., Virology, 24, 388 (1964).

    Article  CAS  Google Scholar 

  17. Fareed, G. C., Sebring, E. D., and Salzman, N. P., J. Biol. Chem., (in the press).

  18. Studier, W. F., J. Mol. Biol., 11, 373 (1965).

    Article  CAS  Google Scholar 

  19. Moses, R. E., Campbell, J. L., Fleischman, R. A., and Richardson, C. C., in Nucleic Acid-protein Interactions-Nucleic Acid Synthesis in Viral Infection (edit. by Ribbons, D. W., Woessner, J. W., and Schultz, T.), 50 (North-Holland, 1972).

    Google Scholar 

  20. Richardson, C. C., Ann. Rev. Biochem., 38, 795 (1969).

    Article  CAS  Google Scholar 

  21. Dürwald, H., and Hoffman-Berling, H., J. Mol Biol., 58, 755 (1971).

    Article  Google Scholar 

  22. Hirt, B., J. Mol. Biol., 26, 365 (1967).

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Laboratory of Biology of Viruses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 20014

    GEORGE C. FAREED & NORMAN P. SALZMAN

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  1. GEORGE C. FAREED
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  2. NORMAN P. SALZMAN
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FAREED, G., SALZMAN, N. Intermediate in SV40 DNA Chain Growth. Nature New Biology 238, 274–277 (1972). https://doi.org/10.1038/newbio238274a0

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