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Isolation of Surface Immunoglobulin from Lymphocytes from Human and Murine Thymus

Nature New Biology volume 235, pages 240–242 (1972)Cite this article

Abstract

CELLS capable of binding radioiodinated antigen occur in lymphocyte populations obtained from foetal and neonatal human thymus1. In accordance with Ehrlich's hypothesis of 1900 (ref. 2), the receptors for antigen on the surface of these cells probably consist of immunoglobulin. A first step in the attainment of chemical proof of the nature of this receptor is the identification of immunoglobulin on the thymus cells. We now report the isolation of immunoglobulin from the surfaces of lymphocytes obtained from murine and neonatal human thymuses. This isolation and identification was made possible by application of a modification3 of the enzymatic iodination procedure of Marchalonis4 which catalysed the iodination of proteins present on the surfaces of living lymphocytes.

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Author information

Author notes

  1. JOHN L. ATWELL: Australian Commonwealth research scholar.

  2. ROBERT E. CONE: Postdoctoral fellow of the Damon Runyon Memorial Fund.

Authors and Affiliations

  1. Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, 3052

    JOHN J. MARCHALONIS, JOHN L. ATWELL & ROBERT E. CONE

Authors
  1. JOHN J. MARCHALONIS
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  2. JOHN L. ATWELL
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  3. ROBERT E. CONE
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MARCHALONIS, J., ATWELL, J. & CONE, R. Isolation of Surface Immunoglobulin from Lymphocytes from Human and Murine Thymus. Nature New Biology 235, 240–242 (1972). https://doi.org/10.1038/newbio235240a0

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