Abstract
RECENT studies have suggested that bovine1–4, rat4 and frog4,5 rhodopsins all have molecular weights close to 28,000. We have now determined the molecular weight of frog (Rana catesbeiana) rhodopsin using several different techniques and obtain a value for the maximum molecular weight of approximately 40,000. Our previous report5 was incorrect because of an error in the calibration of the amino-acid analyser used. These results, together with those of Cavanagh and Wald on cattle rhodopsin6, suggest that the lower values may be in error.
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References
Heller, J., Biochemistry, 7, 2906 (1968).
Schichi, H., Lewis, M. S., Irreverre, F., and Stone, A. L., J. Biol. Chem., 244, 529 (1969).
Shields, J. E., Dinovo, E. C., Henriksen, R. A., Kimbel, R. L., jun., and Millar, P. G., Biochim. Biophys. Acta, 147, 238 (1967).
Heller, J., Biochemistry, 8, 675 (1969).
Bownds, D., and Gaide-Huguenin, A. C., Nature, 225, 870 (1970).
Cavanagh, H. E., and Wald, G., Fed. Proc., 28, 344 (1969).
Bownds, D., Gordon-Walker, A., Gaide-Huguenin, A. C., and Robinson, W., J. Gen. Physiol., 58, 225 (1971).
Matthews, R. G., Hubbard, R., Brown, P. K., and Wald, G., J. Gen. Physiol., 47, 215 (1963).
Zorn, M., and Futterman, S., J. Biol. Chem., 246, 881 (1971).
Bridges, C. D. B., Vis. Research (in the press).
Schichi, H., Biochemistry, 9, 1973 (1970).
Spackman, D. H., Stein, W. H., and Moore, S., Analyt. Chem., 30, 1190 (1958).
Patchornik, A., Lawson, W. B., and Witkop, B., J. Amer. Chem. Soc., 80, 4747 (1958).
Andrews, P., Biochem. J., 96, 595 (1965).
Weber, K., and Osborn, M., J. Biol. Chem., 244, 4406 (1969).
Fish, W. W., Reynolds, J. A., and Tanford, C., J. Biol. Chem., 245, 5166 (1970).
Hubbard, R., J. Gen. Physiol., 37, 381 (1954).
Hall, M. O., Bok, D., and Bacharach, A. D. E., J. Mol. Biol., 45, 397 (1969).
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ROBINSON, W., GORDON-WALKER, A. & BOWNDS, D. Molecular Weight of Frog Rhodopsin. Nature New Biology 235, 112–114 (1972). https://doi.org/10.1038/newbio235112a0
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DOI: https://doi.org/10.1038/newbio235112a0
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