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5′-Terminal Nucleotide Sequence of Reovirus mRNA synthesized in vitro

Nature New Biology volume 235, pages 105–107 (1972)Cite this article

Abstract

THE reovirus genome consists of ten segments of double stranded RNA1–3 that are transcribed in vivo into ten species of single stranded RNA4–6. Eight of these are translated into polypeptides as monocistronic mRNAs7–9. The frequencies of transcription as well as of translation of the individual species of RNA vary considerably6,8, indicating that both transcriptional and translational events are controlled. Since the reovirus genome contains little genetic information not devoted to coding structural polypeptides, the elements of control seem to reside in the nucleotide sequences of the genome RNA segments and mRNA molecules. We have therefore started to examine the nucleotide sequences at the 5′-termini of reovirus mRNA molecules, taking advantage of the fact that reovirus cores transcribe in vitro all ten segments of double stranded RNA into intact mRNA molecules10–14. We report here the sequence of twenty-five nucleotides at the 5′-termini of one or more s mRNA molecules.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina, 27710

    J. L. NICHOLS, A. J. HAY & W. K. JOKLIK

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  1. J. L. NICHOLS
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  2. A. J. HAY
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  3. W. K. JOKLIK
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NICHOLS, J., HAY, A. & JOKLIK, W. 5′-Terminal Nucleotide Sequence of Reovirus mRNA synthesized in vitro. Nature New Biology 235, 105–107 (1972). https://doi.org/10.1038/newbio235105a0

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  • DOI: https://doi.org/10.1038/newbio235105a0

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