Abstract
WE wish to report that reconstituted sperm whale myoglobin prepared by the method of Breslow1 (except that pH 2 was found sufficient to remove all the haem) (I) crystallizes2 in a different habit from those prepared by the method of Rossi-Fanelli et al.3 (II) using haemin of Sigma lot 77B-0220 and our own 57Fe photoporphyrin preparation and the native myoglobin (III). Although all three form type A3 monoclinic prisms, the best developed plane is [001] for II and III, it is [100] for I. There seems to be great interest in reconstituted haemoproteins4,5, so it is important that crystallization habit may be a sensitive test for subtle changes in protein structures.
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References
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DICKINSON, L., CHIEN, J. Crystallization of Reconstituted Sperm Whale Myoglobins. Nature New Biology 234, 107 (1971). https://doi.org/10.1038/newbio234107a0
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DOI: https://doi.org/10.1038/newbio234107a0