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Isolation of Plasmodium berghei (Malaria) Parasites by Ammonium Chloride Lysis of Infected Erythrocytes

Nature New Biology volume 233, pages 260–261 (1971)Cite this article

Abstract

IMMUNOLOGICAL, biochemical and other studies of intra-erythrocytic parasites, such as malaria, would be greatly facilitated if the parasites could be obtained free from erythrocytes. To lyse infected erythrocytes, hypotonic shock1, sonication2, rapid pressure release obtained with the French press3, proteolytic enzyme digestion4,5, saponin6,7 and antibody-induced lysis8,9 have been tried, but these either fail to remove erythrocyte debris completely, or cause structural damage to the organisms.

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Author notes

  1. W. J. MARTIN

    Present address: Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland

Authors and Affiliations

  1. Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 20014

    W. J. MARTIN

  2. Laboratory of Microbial Immunity, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 20014

    J. FINERTY

  3. Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 20014

    A. ROSENTHAL

Authors
  1. W. J. MARTIN
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  2. J. FINERTY
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  3. A. ROSENTHAL
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MARTIN, W., FINERTY, J. & ROSENTHAL, A. Isolation of Plasmodium berghei (Malaria) Parasites by Ammonium Chloride Lysis of Infected Erythrocytes. Nature New Biology 233, 260–261 (1971). https://doi.org/10.1038/newbio233260a0

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