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Structural Changes in Membranes of Synchronized Cells demonstrated by Freeze-cleavage

Nature New Biology volume 233, pages 219–220 (1971)Cite this article

Abstract

FREEZE-CLEAVAGE is a new technique for studying the ultra-structure· of biological membranes, which fractures cell membranes in half, exposing two intramembranous fracture faces1–3: the outer fracture face (OFF) and the inner fracture face (IFF). These fracture faces are partially covered with 70 Å globular particles which are thought to be unique structural components of cell membranes, formed by the association of membrane glycoproteins and lipids4. The 70 Å particles are dynamic structures and rapidly increase in density in the membranes of lymphocytes following exposure to mitogenic plant proteins (Scott and Marchesi, unpublished work).

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Author information

Authors and Affiliations

  1. Laboratory of Experimental Pathology, National Institute of Arthritis and Metabolic Disease, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20014

    ROBERT E. SCOTT & RAYMOND L. CARTER

  2. Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20014

    WILLIAM R. KIDWELL

Authors
  1. ROBERT E. SCOTT
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  2. RAYMOND L. CARTER
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  3. WILLIAM R. KIDWELL
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SCOTT, R., CARTER, R. & KIDWELL, W. Structural Changes in Membranes of Synchronized Cells demonstrated by Freeze-cleavage. Nature New Biology 233, 219–220 (1971). https://doi.org/10.1038/newbio233219a0

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  • DOI: https://doi.org/10.1038/newbio233219a0

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