Abstract
VARIOUS substances isolated from plants cause animal cells to clump. Several of these lectins1 preferentially agglutinate cells which have been transformed spontaneously or by chemicals or viruses2–7. The best known lectins of this class are concanavalin A (Con A) isolated from jack beans8 and wheat germ agglutinin4, which seem to bind to carbohydrate groups on the cell surface. The determinants recognized by the lectins seem to be N-acetyl-D-glucosamine for WGA4 and probably α-methyl-D-glucopyranoside for Con A6.
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References
Boyd, W. C., An Introduction to Immunochemical Specificity (Interscience, New York, 1965).
Ambrose, E. J., Dudgeon, J. A., Easty, D. M., and Easty, J. C., Exp. Cell. Res., 24, 220 (1961).
Aub, J. C., Tieslau, C., and Lankester, A., Proc. US Nat. Acad. Sci., 50, 613 (1963).
Burger, M. M., and Goldberg, A. R., Proc. US Nat. Acad. Sci., 57, 359 (1967).
Pollack, R. E., and Burger, M. M., Proc. US Nat. Acad. Sci., 62, 1074 (1969).
Inbar, M., and Sachs, L., Proc. US Nat. Acad. Sci., 63, 1418 (1969).
Inbar, M., and Sachs, L., Nature, 223, 710 (1969).
Sumner, J. B., Howell, S. F., and Zeissig, A., Science, 82, 65 (1935).
Burger, M. M., Proc. US Nat. Acad. Sci., 62, 994 (1969).
Burger, M. M., Nature, 227, 170 (1970).
Burger, M. M., and Noonan, K. D., Nature, 228, 512 (1970).
Fox, T. O., Sheppard, J. R., and Burger, M. M., Proc. US Nat. Acad. Sci., 69, 244 (1971).
Agrawal, B. B. L., and Goldstein, I. J., Biochem. J., 96, 23C (1965).
Singer, T. P., and Hofstee, B. H. J., Arch. Biochem. Biophys., 228, 229 (1948).
Marchalonis, J. J., Biochem. J., 113, 299 (1969).
Hirt, B., J. Mol. Biol., 26, 365 (1967).
Eckhart, W., Dulbecco, R., and Burger, M. M., Proc. US Nat. Acad. Sci., 68, 283 (1971).
Rabinowitz, Z., and Sachs, L., Nature, 225, 136 (1970).
Ozanne, B., and Sambrook, J., in The Biology of Oncogenic Viruses, 248 (North Holland, Amsterdam, 1971).
Liske, R., and Franks, D., Nature, 217, 861 (1968).
Aub, J. C., Sanford, B. H., and Cote, M. N., Proc. US Nat. Acad. Sci., 54, 396 (1965).
Aub, J. C., Sanford, B. H., and Wang, L. H., Proc. US Nat. Acad. Sci., 54, 400 (1965).
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OZANNE, B., SAMBROOK, J. Binding of Radioactively Labelled Concanavalin A and Wheat Germ Agglutinin to Normal and Virus-transformed Cells. Nature New Biology 232, 156–160 (1971). https://doi.org/10.1038/newbio232156a0
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DOI: https://doi.org/10.1038/newbio232156a0
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