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Saying a firm ‘no’ to DNA re-replication

Nature Cell Biology volume 1, pages E175–E177 (1999)Cite this article

Abstract

Yeast cells exclude the DNA-replication-initiation factor Mcm4 from the nucleus during the S and G2 phases of the cell cycle, when active replication ‘origins’ must not reassemble. This exclusion is dependent on cyclin-dependent kinases and provides a general mechanism for preventing re-replication of DNA in a single cell cycle.

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Figure 1: Cyclin-dependent kinase (CDK)-dependent localization of Mcms.
Figure 2: Redistribution of prereplicative-complex components between nucleus and cytoplasm during S/G2 phases of the cell cycle in different organisms.

References

  1. Labib, K., Diffley, J. F. X. & Kearsey, S. E. Nature Cell Biol. 1, 415– 422 (1999).

    Article  CAS  Google Scholar 

  2. Tye, B. K. Annu. Rev. Biochem. 68, 649–686 (1999).

    Article  CAS  Google Scholar 

  3. Tada, S., Chong, J. P. J., Mahbubani, H. M. & Blow, J. J. Curr. Biol. 9, 211–214 ( 1999).

    Article  CAS  Google Scholar 

  4. Blow, J. J. & Laskey, R. A. Nature 332, 546–548 (1988).

    Article  CAS  Google Scholar 

  5. Hennessy, K. M., Clark, C. D. & Botstein, D. Genes Dev. 4, 2252– 2263 (1990).

    Article  CAS  Google Scholar 

  6. Kimura, H., Ohtomo, T., Yamaguchi, M., Ishii, A. & Sugimoto, K. Genes Cells 1, 977–993 (1996).

    Article  CAS  Google Scholar 

  7. Young, M. R., Suzuki, K., Yan, H., Gibson, S. & Tye, B. K. Genes Cells 2, 631– 643 (1997).

    Article  CAS  Google Scholar 

  8. Broek, D., Bartlett, R., Crawford, K. & Nurse, P. Nature 349, 388–393 ( 1991).

    Article  CAS  Google Scholar 

  9. Kimura, H., Nozaki, N. & Sugimoto, K. EMBO J. 13, 4311– 4320 (1994).

    Article  CAS  Google Scholar 

  10. Saha, P. et al. Mol. Cell. Biol. 18, 2758– 2767 (1998).

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Biochemistry, CRC Chromosome Replication Research Group, University of Dundee, MSI/WTB Complex, Dow Street, Dundee , DD1 5EH, UK

    J. Julian Blow & Tatyana A. Prokhorova

Authors
  1. J. Julian Blow
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  2. Tatyana A. Prokhorova
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Correspondence to J. Julian Blow.

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Blow, J., Prokhorova, T. Saying a firm ‘no’ to DNA re-replication. Nat Cell Biol 1, E175–E177 (1999). https://doi.org/10.1038/15611

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