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Synaptic vesicle retrieval: still time for a kiss

Nature Cell Biology volume 4, pages E245–E248 (2002)Cite this article

After exocytosis, synaptic vesicles are reformed by slow clathrin-mediated endocytosis. However, evidence also supports the existence of faster retreival mechanisms in neurons, including 'kiss-and-run', where vesicles fuse only partially with the presynaptic membrane before being retrieved. New insights in synaptic vesicle dynamics have been obtained from vesicle imaging and from studies with mutant animals. Recently, measurements of capacitance changes induced by the fusion of single synaptic vesicles in synapses corroborate the hypothesis that kiss-and-run operates in neurons. Here, we review the evidence supporting fast vesicle retrieval and evaluate its role in neurotransmitter release.

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Figure 1: FM dye properties in the unloading paradigm.
Figure 2: Kiss and run in neurons.

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Acknowledgements

We thank E. Seto, A. Fayyazuddin, J. Hounsgaard and P. Saggau for their comments on the manuscript. O.K is supported by the Novo Nordisk Foundation and the Lundbeck Foundation. H.J.B. is supported by the Howard Hughes Medical Institute.

Author information

Authors and Affiliations

  1. Division of Neurophysiology, Department of Medical Physiology, University of Copenhagen, Copenhagen, DK-2200, Denmark

    Ole Kjaerulff

  2. Program in Developmental Biology, Baylor College of Medicine, Houston, 77030, TX, USA

    Patrik Verstreken

  3. Department of Molecular and Human Genetics, Division of Neuroscience, Program in Developmental Biology and the Howard Hughes Medical Institute, Baylor College of Medicine, Houston, 77030, TX, USA

    Hugo J. Bellen

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  1. Ole Kjaerulff
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  2. Patrik Verstreken
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  3. Hugo J. Bellen
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Kjaerulff, O., Verstreken, P. & Bellen, H. Synaptic vesicle retrieval: still time for a kiss. Nat Cell Biol 4, E245–E248 (2002). https://doi.org/10.1038/ncb1102-e245

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