Abstract
The actin cytoskeleton orders cellular space and transduces many of the forces required for morphogenesis. Here we combine genetics and cell biology to identify genes that control the polarized distribution of actin filaments within the Drosophila follicular epithelium. We find that profilin and cofilin regulate actin-filament formation throughout the cell cortex. In contrast, CAP—a Drosophila homologue of Adenylyl Cyclase Associated Proteins—functions specifically to limit actin-filament formation catalysed by Ena at apical cell junctions. The Abl tyrosine kinase also collaborates in this process. We therefore propose that CAP, Ena and Abl act in concert to modulate the subcellular distribution of actin filaments in Drosophila.
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Acknowledgements
We thank Zak Wills, Jack Bateman, David Van Vactor and David Bilder for help throughout this project; T. Schupbach, K. Gunsalus, D. St Johnston and the Bloomington Stock Center for Drosophila stocks, and D. Brenton, M. Hoffmann and E. Knust for antibodies; and B. Stronach, S. Noselli, D. Bilder, M. Schober, D. Van Vactor, A. Kiger and F. Gertler for their comments on the manuscript. B.B. was supported by EMBO, HFSP and HHMI, and N.P. is an investigator of the Howard Hughes Medical Institute.
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Baum, B., Perrimon, N. Spatial control of the actin cytoskeleton in Drosophila epithelial cells. Nat Cell Biol 3, 883–890 (2001). https://doi.org/10.1038/ncb1001-883
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DOI: https://doi.org/10.1038/ncb1001-883
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