Fixed rat kidney cells were labelled using markers for podocyte bodies and primary processes (blue) and podocyte nuclei (brown). Optical slices were collected using two-photon fluorescence microscopy (a Bio-Rad MRC1024 laser scanning confocal two-photon system with a Nikon microscope and a Spectra-Physics laser) and were rendered in three dimensions using Voxx software. Bar, 50 μm.