Abstract
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of ∼50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We have demonstrated not only that an antibody-tagged label is stable enough to be recorded in this microscopy mode, but also that subdiffraction resolution can be obtained using a standard immunofluorescence preparation.
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References
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Acknowledgements
We thank Lars Kastrup for valuable discussions and the German Ministry for Research and Education (BMBF) for partial support.
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Dyba, M., Jakobs, S. & Hell, S. Immunofluorescence stimulated emission depletion microscopy. Nat Biotechnol 21, 1303–1304 (2003). https://doi.org/10.1038/nbt897
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DOI: https://doi.org/10.1038/nbt897
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