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Use of Living Columns to Select Specific Phage Antibodies

Bio/Technology volume 11, pages 1565–1569 (1993)Cite this article

Abstract

Here we demonstrate that it is possible to confront two recombinant microorganisms in order to select one using the other. We have shown that an epitope derived from p21ras expressed within the outer membrane protein, LamB, can be recognized both by the monoclonal antibody Y13-259, as well as the single chain Fv fragment derived from it. This specificity, which is maintained when the Y13-259 single chain Fv is expressed as a fusion protein with the phage fd gene 3 protein, has allowed us to use the living column of LamB-ras to purify Y13-259 phage from a background of non-binding phage, even at dilutions as high as 10 phage in 1010 irrelevant phage.

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Author notes

  1. Andrew Bradbury and Antonino Cattaneo: Corresponding authors.

Authors and Affiliations

  1. SISSA, Biophysics Sector, via Beirut 2-4, 34013, Trieste, Italy

    Andrew Bradbury & Antonino Cattaneo

  2. CNR Istituto di Neurobiologia, via Karl Marx 15, 00156, Roma, Italy

    Thomas Werge & Antonino Cattaneo

  3. Società Italiana per la Ricerca Scientifica, Area di Ricerca, Padriciano 99, 34012, Trieste, Italy

    Andrew Bradbury & Lidija Persic

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  3. Thomas Werge
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  4. Antonino Cattaneo
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Bradbury, A., Persic, L., Werge, T. et al. Use of Living Columns to Select Specific Phage Antibodies. Nat Biotechnol 11, 1565–1569 (1993). https://doi.org/10.1038/nbt1293-1565

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