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Purification and Characterization of Human Interleukin–1 Expressed in Escherichia coli

Bio/Technology volume 4, pages 1078–1082 (1986)Cite this article

Abstract

Two forms of human interleukin–1 have been expressed in E. coli. Acid extraction of the cellular suspensions solubilizes rIL–1α and rIL–1β from inclusion bodies while precipitating most of the E. coli proteins. This leads to a facile purification scheme that provides sufficient quantities of highly active IL–1 to undertake biological characterization of these important immunoregulatory molecules.

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Authors and Affiliations

  1. Department of Protein Chemistry, Immunex Corporation, 51, University Street, Seattle, WA, 98101

    Shirley R. Kronheim, Carl J. March, Paula J. Glackin, Janet E. Merriam & Thomas P. Hopp

  2. Department of Molecular Biology, Immunex Corporation, 51, University Street, Seattle, WA, 98101

    Michael A. Cantrell, Michael C. Deeley, Dirk M. Anderson, Toby Hemenway & David Cosman

Authors
  1. Shirley R. Kronheim
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  2. Michael A. Cantrell
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  3. Michael C. Deeley
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  4. Carl J. March
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  5. Paula J. Glackin
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  6. Dirk M. Anderson
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  7. Toby Hemenway
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  8. Janet E. Merriam
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  9. David Cosman
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  10. Thomas P. Hopp
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Kronheim, S., Cantrell, M., Deeley, M. et al. Purification and Characterization of Human Interleukin–1 Expressed in Escherichia coli. Nat Biotechnol 4, 1078–1082 (1986). https://doi.org/10.1038/nbt1286-1078

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  • DOI: https://doi.org/10.1038/nbt1286-1078

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