On page 1314, Zhang et al. describe a way to efficiently clone large target sequences in a single step. First an appropriate bacterial host containing a highly efficient phage recombination system is transformed with the target DNA along with a PCR-generated fragment that contains a replication origin, a selectable antibiotic resistance marker, and short homologous ends to the target DNA. The phage homologous recombination machinery then transfers the target DNA into the linear cloning vector.
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Sinclair, M. Technical Reports. Nat Biotechnol 18, 1233 (2000). https://doi.org/10.1038/82319
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DOI: https://doi.org/10.1038/82319