On page 1314, Zhang et al. describe a way to efficiently clone large target sequences in a single step. First an appropriate bacterial host containing a highly efficient phage recombination system is transformed with the target DNA along with a PCR-generated fragment that contains a replication origin, a selectable antibiotic resistance marker, and short homologous ends to the target DNA. The phage homologous recombination machinery then transfers the target DNA into the linear cloning vector.