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Receptor-Affinity Chromatography: A One-Step Purification for Recombinant Interleukin-2

Bio/Technology volume 5, pages 1195–1198 (1987)Cite this article

Abstract

We have developed a receptor–affinity method for the rapid and selective purification of recombinant interleukin–2 (rIL–2) based on the specific interactions between a matrix–bound receptor and its soluble protein ligand. Receptor–affinity purified rIL–2 contains only a highly active monomeric form of the lymphokine in contrast to immunoaffinity chromatography where several molecular forms of rIL–2, with varying degrees of biological activity, are recovered.

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Author notes

  1. Joan E. Fredericks, Cynthia Smith and Philip C. Familletti: Molecular Genetics Department, Roche Research Center, Hoffmann-LaRoche Inc., Nutley, NJ 07110.

  2. Pascal Bailon: To whom correspondence should be addressed.

Authors and Affiliations

  1. Department of Protein Biochemistry, Roche Research Center, Hoffmann-LaRoche Inc., Nutley, NJ, 07110

    Pascal Bailon, David V. Weber, Ronald F. Keeney, Joan E. Fredericks, Cynthia Smith, Philip C. Familletti & John E. Smart

Authors
  1. Pascal Bailon
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  2. David V. Weber
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  3. Ronald F. Keeney
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  4. Joan E. Fredericks
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  5. Cynthia Smith
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  6. Philip C. Familletti
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  7. John E. Smart
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Bailon, P., Weber, D., Keeney, R. et al. Receptor-Affinity Chromatography: A One-Step Purification for Recombinant Interleukin-2. Nat Biotechnol 5, 1195–1198 (1987). https://doi.org/10.1038/nbt1187-1195

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