Abstract
The ability to isolate functional populations of hematopoietic progenitor cells is important to the process of hematopoietic cell transplantation and to the understanding of hematopoietic cell biology in health and disease. We show that a subpopulation of human bone marrow hematopoietic cells bearing the pan-hematopoietic antigen CD34 also binds galactose-conjugated proteins. This lectin-positive sub-population represents approximately 0.1 to 0.5% of the total bone marrow cells, and contains 100% of the hematopoietic progenitor cells. The galactose-binding lectin on these cells is specific for this sugar. Additionally, highly proliferative hematopoietic progenitor cells with very primitive phenotypes, including a newly identified progenitor cell that produces multiple lineages, express this lectin.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Long, M.W. 1992. Blood cell cytoadhesion molecules. Exp. Hematol. 20: 288–301.
Sutherland, D.R. and Keating, A. 1992. The CD34 antigen: structure, biology, and potential clinical applications. [Review]. J. Hematother. 1: 115–129.
Murray, L.J., Chen, B., Galy, A., Chen, S., Tushinski, R., Uchida, N. et al. 1995. Enrichment of human hematopoietic stem cell activity in the CD34+Thy-1 +Lin-subpopulation from mobilized peripheral blood. Blood 85: 368–378.
Hardy, C.L., Matsuoka, T. and Tavassoli, M. 1991. Distribution of homing protein on hemopoietic stromal and progenitor cells. Exp. Hematol. 19: 968–972.
Aizawa, S. and Tavassoli, M. 1987. In vitro homing of hemopoietic stem cells is mediated by a recognition system with galactosyl and mannosyl specificities. Proc. Natl. Acad. Sci. USA 84: 4485–4489.
Sullenberger, B.A., Petitt, M.S., Chong, P., Long, M.W. and Wicha, M.S. 1995. Murine granulocytic cell adhesion to bone marrow hemonectin is mediated by mannose and galactose. Blood 86: 135–140.
Berardi, A.C., Wang, A., Levine, J.D., Lopez, P. and Scadden, D.T. 1995. Functional isolation and characterization of human hematopoietic stem cells. Science 267: 104–108.
Van Epps, D.E., Bender, J., Schilling, M., Smith, S., Unverzagt, K., Law, P. and Burgess, J. 1994. Harvesting, charachterization, and culture of CD34+ cells from human bone marrow peripheral blood and cord blood. Blood Cells 20: 411–423.
Rusten, L., Jacobsen, S., Kaalhus, J., Veilby, O., Funderud, S. and Smeland, E. 1994. Functional differences between CD38- and DR- subfractions of CD34+ bone marrow cells. Blood 84: 1473–1481.
Nagler, A., Peacock, M., Tantoco, M., Lamons, D., Okarma, T. and Okrongly, D. 1994. Red blood cell depletion and enrichment of CD34+ hematopoietic progenitor cells from human umbilical cord blood using soybean agglutinin and CD34 immunoselection. Exp. Hematol. 22: 1134–1140.
Lansdorp, P.M., Sutherland, H.J. and Eaves, C.J. 1990. Selective expression of CD45 isoforms on functional subpopulations of CD34+ hemopoietic cells from human bone marrow. J. Exp. Med. 172: 363–366.
Craig, W., Kay, R., Cutler, R.L. and Lansdorp, P.M. 1993. Expression of Thy-1 on human hematopoietic progenitor cells. J. Exp. Med. 177: 1331–1342.
Long, M.W., Hutchinson, R.J., Gragowski, L.L., Heffner, C.H. and Emerson, S.G. 1988. Synergistic regulation of human megakaryocyte development. J. Clin. Invest. 82: 1779–1786.
de Wynter, E.A., Coutinho, L.H., Pei, X., Marsh, J.C., Hows, J., Luft, T., and Testa, N.G. 1995. Comparison of purity and enrichment of CD34* cells from bone marrow, umbilical cord and peripheral blood (primed for apheresis) using five separation systems. Stem Cells 13: 532.
McNeice, I.K., Stewart, F.M., Deacon, D.M., Temeles, D.S., Zsebo, K.M., Clark, S.C. and Quesenberry, P.J. 1989. Detection of a human CFC with a high prolif-erative potential. Blood 74: 609–612.
Long, M.W., Briddell, R., Walter, A.W., Bruno, E. and Hoffman, R. 1992. Human hematopoietic stem cell adherence to cytokines and matrix molecules. J. Clin. Invest. 90: 251–255.
Carow, C.E., Hangoc, G. and Broxmeyer, H.E. 1993. Human multipotential progenitor cells have extensive replating capacity for secondary CFU-GEMM: an effect enhanced by cord blood plasma. Blood 81: 942–949.
Huyhn, A., Dommergues, M., Izac, B., Croisille, L., Katz, A., Vainchenker, W. and Coulombel, L. 1995. Characterization of hematopoietic progenitors from human yolk sacs and embryos. Blood 86: 4474–4485.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pipia, G., Long, M. Human hematopoietic progenitor cell isolation based on galactose-specific cell surface binding. Nat Biotechnol 15, 1007–1011 (1997). https://doi.org/10.1038/nbt1097-1007
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/nbt1097-1007