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Cloning and Expression of the Gene for Pro-urokinase in Escherichia coli

Bio/Technology volume 3, pages 923–929 (1985)Cite this article

Abstract

An Escherichia coli library containing cDNA sequences derived from mRNA of human pharnygeal carcinoma cells was constructed using the plasmid pBR322. The library was screened with a set of 8 deoxyoligonucleotides, 14 bases in length, which collectively code for the amino acid sequence …Met-Tyr-Asn-Asp-Pro… found in human urokinase (u-PA). A plasmid containing an ∼1700 base pair cDNA insert coding for the carboxy terminal sequence of u-PA was identified. The 5′ region was obtained by priming poly(A) mRNA with a DNA fragment derived from the initial clone. An expression vector was constructed which directs the synthesis of pro u-PA in E. coli.

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Author notes

  1. William E. Holmes

    Present address: Center for Thrombosis and Vascular Research, Department of Medical Research, University of Leuven, B-3000, Leuven, Belgium

  2. Herbert L. Heyneker

    Present address: Genencor, Inc., 180 Kimball Way, South San Francisco, CA, 94080, USA

  3. Wolfgang A. Guenzler and Gerd J. Steffens: Gruenenthal, GmbH Zieglerstrasse 6, 5100 Aachen, Federal Republic of Germany.

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  1. Genentech, Inc., 460 Pt. San Bruno Blvd., South San Francisco, CA, 94080, USA

    Diane Pennica, Mike Blaber & Michael W. Rey

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Holmes, W., Pennica, D., Blaber, M. et al. Cloning and Expression of the Gene for Pro-urokinase in Escherichia coli. Nat Biotechnol 3, 923–929 (1985). https://doi.org/10.1038/nbt1085-923

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