Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Research Paper
  • Published:

Detection of Antibodies to Human T-Cell Lymphotropic Virus-III (HTLV-III) with an Immunoassay Employing a Recombinant Escherichia coli-Derived Viral Antigenic Peptide

Bio/Technology volume 3, pages 905–909 (1985)Cite this article

Abstract

A peptide containing 82 amino acid residues encoded by a gene segment in the env-lor region of HTLV-III has been produced in large quantities in Escherichia coli. Solid phase immunoassays employing the recombinant peptide as an antigenic adsorbent can reproducibly detect antibodies in sera of patients with HTLV-III infection. In several representative serum panels, the assay detected the presence of antibodies in 131 of 132 sera from patients with AIDS or AIDS-related complex (ARC), and in none of 471 normal blood donors. These results indicate that a sensitive, specific and quantitative immunoassay for HTLV-III infection can be developed with a recombinant viral antigen as the immunoreactive agent.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Popovic, M., Sarngadharan, M.G., Read, E. and Gallo, R.C. 1984. Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. Science 224: 497–500.

    Article  CAS  Google Scholar 

  2. Gallo, R.C., Salahuddin, S.Z., Popovic, M., Shearer, G.M., Kaplan, M., Haynes, B.F., Parker, T.J., Redfrield, R., Oleske, J., Safai, B., White, G., Foster, P. and Markham, P.D. 1984. Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS. Science 224: 500–503.

    Article  CAS  Google Scholar 

  3. Barre-Sinoussi, F., Chermann, J.C., Rey, F., Nugeyre, M.T., Chamaret, S., Gruest, J., Danguet, C., Axler-Blin, C., Vezinet-Brun, F., Rouzioux, C., Rozenbaum, W. and Montagnier, L. 1983. Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). Science 220: 868–871.

    Article  CAS  Google Scholar 

  4. Levy, J.A., Hoffman, A.D., Kramer, S.M., Landis, J.A., Shimabukuro, J.M. and Oshiro, L.S. 1984. Isolation of lymphocytopathic retroviruses from San Francisco patients with AIDS. Science 225: 840–842.

    Article  CAS  Google Scholar 

  5. Sarngadharan, M.G., Popovic, M., Bruch, L., Schupbach, J. and Gallo, R.C. 1984. Antibodies reactive with T-lymphotropic viruses (HTLV-III) in the serum of patients with AIDS. Science 224: 506–508.

    Article  CAS  Google Scholar 

  6. Gazzard, B.G., Shanson, D.C., Farthing, C., Lawrence, A.G., Tedder, R.S., Cheingsong-Popov, R., Dalgleish and Weiss, R.A. 1984. Clinical findings and serological evidence of HTLV-III infection in homosexual contacts of patients with AIDS and persistent generalized lymphadenopathy in London. Lancet ii: 480–483.

    Article  Google Scholar 

  7. Cheingsong-Popov, R., Weiss, R.A., Tedder, R.S., Shandon, D.C., Jeffries, D.J., Ferns, R.B., Weller, I.V.D., Mitton, S., Adler, M.W., Farthing, C., Lawrence, A.G., Gazzard, B.G., Weber, J., Harris, J.R.W., Pinching, A.J., Craske, J. and Barbara, J.A.J. 1984. Prevalence of antibody to human T-lymphotropic virus type III in AIDS and AIDS-risk patients in Britain. Lancet ii: 476–480.

    Google Scholar 

  8. Kitchen, L.W., Barin, F., Sullivan, J.L., McLane, M.F., Brettler, D.B., Levine, P.H. and Essex, M. 1984. Aetiology of AIDS-antibodies to human T-cell leukaemia virus (type III) in haemophiliacs. Nature 312: 367–369.

    Article  CAS  Google Scholar 

  9. Safai, B., Sarngadharan, M.G., Groopman, J.E., Arnett, K., Popovic, M., Sliski, A., Schupback, J. and Gallo, R.C. 1984. Seroepidemiological studies of human T-lymphotropic retrovirus type III in acquired immunodeficiency syndrome. Lancet i: 1438–1439.

    Article  Google Scholar 

  10. Kalymaraman, V.S., Cabradilla, C.D., Getchell, J.P., Nanayanan, R., Braff, E.H., Cherman, J.-C., Barre-Sinoussi, F., Montagnier, L., Spira, T.J., Kaplan, J., Fishbein, D., Jaffe, H.W., Curran, J.W. and Francis, D.P. 1984. Antibodies to the core protein of lymphodenopathy-associated virus (LAV) in patients with AIDS. Science 225: 321.

    Article  Google Scholar 

  11. Neurath, A.R., Strick, N., Sproul, P., Baker, L., Rubinstein, P., Stevens, C.E., Taylor, P., Gallo, R.C., Gold, J.W.M., Lee, Y.S. and Nilsen, T. 1985. Radioimmunoassay and enzyme-linked immunoassay of antibodies to the core protein (P24) of human T-lymphotropic virus (HTLV-III). J. Immunol. Med. 11: 75–86.

    CAS  Google Scholar 

  12. Brun-Vezinet, F., Rouzioun, C., Barre-Sinoussi, F., Klatzmann, D., Saimot, A.G., Rozenbaum, W., Christol, D., Gluckmann, J.C., Montagnier, L. and Chermann, J.C. 1984. Detection of IgG antibodies to lymphadenopathy-associated virus in patients with AIDS or lymphadenopathy syndrome. Lancet i: 1253–1256.

    Article  Google Scholar 

  13. Laurence, J., Brun-Vezinet, F., Schutzer, S.E., Rouzioux, C., Klatzmann, D., Barre-Sinoussi, F., Chermann, J.-C. and Montagnier, L. 1984. Lymphadenopathy-associated viral antibody in AIDS: immune correlation and definition of a carrier state. N. Eng. J. Med. 311: 1269–1273.

    Article  CAS  Google Scholar 

  14. Spira, T.J., Des Jardis, D.C., Marmor, M., Yancovitz, S., Friedman, S., Garber, J., Cohen, H., Cabradilla, C. and Kalyanaraman, V.C. 1984. Prevalence of antibody to lymphodenopathy-associated virus among drug-detoxification patients in New York. N. Eng. J. Med. 311: 467–468.

    Article  CAS  Google Scholar 

  15. Tsoukas, C., Gervais, F., Shuster, J., Gold, P., O'Shaughnessy, M. and Robert-Guroff, M. 1984. Association of HTLV-III antibodies and cellular immune status of hemophiliacs. N. Eng. J. Med. 311: 1514–1516.

    Article  CAS  Google Scholar 

  16. Weiss, S.H., Goedert, J.J., Sarngadharan, M.G., Bodner, A.J., Gallo, R.C. and Blattner, W.A. 1985. Screening test for HTLV-III (AIDS agent) antibodies. JAMA 253: 221–225.

    Article  CAS  Google Scholar 

  17. Weiss, R.A., Clapham, P.R., Cheingsong-Popov, R., Dalgleish, A.G., Carne, C.A., Weller, I.V.D. and Tedder, R.S. 1985. Neutralization of human T-lymphotropic virus type III by sera of AIDS and AIDS-risk patients. Nature 316: 69–72.

    Article  CAS  Google Scholar 

  18. Robert-Guroff, M., Brown, M. and Gallo, R.C. 1985. HTLV-III-neutralizing antibodies in patients with AIDS and AIDS-related complex. Nature 316: 72–74.

    Article  CAS  Google Scholar 

  19. Chang, N.T., Chanda, P.K., Barone, A.D., McKinney, S., Rhodes, D.P., Tam, S.H., Shearman, C.W., Huang, J., Chang, T.W., Gallo, R.C. and Wong-Staal, F. 1985. Expression in Escherichia coli of open reading frame gene segments of HTLV-III. Science 228: 93–96.

    Article  CAS  Google Scholar 

  20. Chang, N.T., Huang, J., Ghrayeb, J., McKinney, S., Chanda, P.K., Chang, T.W., Putney, S., Sarngadharan, M.G., Wong-Staal, F. and Gallo, R.C. 1985. An HTLV-III peptide produced by recombinant DNA is immunoreactive with sera from patients with AIDS. Nature 315: 151–154.

    Article  CAS  Google Scholar 

  21. Crowl, R., Ganguly, K., Gordon, M., Conroy, R., Schaber, M., Kramer, R., Shaw, G., Wong-Staal, F. and Reddy, E.P. 1985. HTLV-III env gene products synthesized in E. coli are recognized by antibodies present in the sera of AIDS patients. Cell 41: 979–986.

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Flossie Wong-Staal and Robert C. Gallo: Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, MD 20205

Authors and Affiliations

  1. Centocor, Inc., 244 Great Valley Parkway, Malvern, PA, 19355

    Tse Wen Chang, Ikunoshin Kato, Sam McKinney, Pranab Chanda, Anthony D. Barone & Nancy T. Chang

Authors
  1. Tse Wen Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ikunoshin Kato
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sam McKinney
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pranab Chanda
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Anthony D. Barone
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Flossie Wong-Staal
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Robert C. Gallo
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Nancy T. Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chang, T., Kato, I., McKinney, S. et al. Detection of Antibodies to Human T-Cell Lymphotropic Virus-III (HTLV-III) with an Immunoassay Employing a Recombinant Escherichia coli-Derived Viral Antigenic Peptide. Nat Biotechnol 3, 905–909 (1985). https://doi.org/10.1038/nbt1085-905

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt1085-905

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing