Abstract
The filamentous fungus Trichoderma reesei produces a number of inducible cellulolytic enzymes. By differential hybridization of cDNA probes made from induced and repressed messenger RNA populations to a λ phage gene bank, we have isolated a number of genes strongly expressed during production of cellulolytic enzymes. We have shown by hybrid messenger selection and partial DNA sequencing that one of the clones contains a gene coding for the major cellulolytic enzyme of T. reesei, cellobiohydrolase I.
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References
Fägerstam, L.G. 1981. Cellulases from Trichoderma reesei QM9414: Enzymatic and structural properties. Doctoral Thesis, University of Uppsala, Sweden 1981.
Pettersson, G., Fägerstam, L., Bhikhahhai, R. and Leandoer, K. 1981. The cellulase complex of Trichoderma reesei QM9414. Int. Symp. on Wood and Pulping Chem. 3: 39–42.
Salovuori, I., Teeri, T., Niku-Paavola, M.-L. and Knowles, J. 1983. Submitted for publication.
Nummi, M., Niku-Paavola, M.-L., Lappalainen, A., Enari, P.-M. and Racenio, V. 1983. Cellobiohydrolase from Trichoderma reesei. Biochem J. In press.
Bailey, M.J. and Nevalainen, K.M.H. 1981. Induction, isolation and testing of stable Trichoderma reesei mutants with improved production of solubilizing cellulase. Enzyme Microb. Technol. 3: 153–157.
Mandels, M., Weber, J. and Parizek, R. 1971. Enhanced cellulasc production by a mutant of Trichoderma viride. Appl. Microbiol. 21: 152–154.
Karn, J., Brenner, S., Barnett, L. and Cesaveni, G. 1980. Novel bacteriophage λ cloning vector. Proc. Natl. Acad. Sci. 77: 5172–5176.
Messing, J., Crea, R. and Seeburg, P.H. 1981. A system for shotgun DNA sequencing. Nucleic Acids Res. 9: 309–321.
Hautala, J.A., Corner, B.H., Jacobson, J.N., Patel, G.L. and Giles, N.H. 1977. Isolation and characterization of nuclei from Neurospora crassa. J. Bacteriol. 130: 704–713.
Ohi, S. and Short, J. 1980. A general procedure for preparing messenger RNA from eukaryotic cells without using phenol. J. Appl. Microbiol. 2: 398–413.
Aviv, H. and Leder, P. 1972. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic add cellulose. Proc. Natl. Acad. Sci. 69: 1408–1412.
Clevell, D.B. 1972. Nature of Cal Ei plasmid replication by Escherichia coli in the presence of chloramphenicol. J. Bact. 110: 667–676.
Holmes, D.S. and Quigley, M. 1981. A rapid boiling method for the preparation of bacterial plasmids. Anal. Biochem. 14: 193–197.
Blattner, F.R., Williams, B.G., Blechl, A.E., Denniston-Thompson, C., Faber, H.E., Furlong, L.-A., Grunwell, D.J., Kiefer, D.O., Moore, D.D., Sheldon, E.L. and Smithies, O. 1977. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning. Science 196: 161–169.
Maniatis, T., Fritsch, E.F. and Sambrook, J. 1982. Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Heidecker, G., Messing, J., and Gronenborn, B. 1980. A versatile primer for DNA sequencing in the M13 mp2 cloning system. Gene 10: 69–73.
Efstradiatis, A., Kafatos, F.C., Maxam, A.M. and Maniatis, T. 1976. Enzymatic in vitro synthesis of globin genes. Cell 7: 279–288.
Laemmli, U. 1970. Cleavage of structural proteins during the assembly of bacteriophage T4. Nature 227: 680–685.
Sanger, F., Nicklen, S. and Coulsen, A.R. 1977. DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. 74: 5463–5467.
Benton, W.D. and Davis, R.W. 1977. Screening δgt, recombinant clones by hybridization to single plaques in situ. Science 196: 180–182.
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Teeri, T., Salovuori, I. & Knowles, J. The Molecular Cloning of the Major Cellulase Gene from Trichoderma Reesei. Nat Biotechnol 1, 696–699 (1983). https://doi.org/10.1038/nbt1083-696
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DOI: https://doi.org/10.1038/nbt1083-696
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