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The Molecular Cloning of the Major Cellulase Gene from Trichoderma Reesei

Abstract

The filamentous fungus Trichoderma reesei produces a number of inducible cellulolytic enzymes. By differential hybridization of cDNA probes made from induced and repressed messenger RNA populations to a λ phage gene bank, we have isolated a number of genes strongly expressed during production of cellulolytic enzymes. We have shown by hybrid messenger selection and partial DNA sequencing that one of the clones contains a gene coding for the major cellulolytic enzyme of T. reesei, cellobiohydrolase I.

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Teeri, T., Salovuori, I. & Knowles, J. The Molecular Cloning of the Major Cellulase Gene from Trichoderma Reesei. Nat Biotechnol 1, 696–699 (1983). https://doi.org/10.1038/nbt1083-696

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