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Snapshots of kinase activities

Nature Biotechnology volume 27, pages 912–913 (2009)Cite this article

A mass spectrometry–based strategy facilitates sensitive and rapid detection of kinase activities in small amounts of cell lysate.

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Figure 1: Single-reaction KAYAK enables kinase activities to be monitored in different biological contexts.

References

  1. Kubota, K. et al. Nat. Biotechnol. 27, 933–940 (2009).

    Article  CAS  Google Scholar 

  2. Yu, Y. et al. Proc. Natl. Acad. Sci. USA 106, 11606–11611 (2009).

    Article  CAS  Google Scholar 

  3. Villen, J., Beausoleil, S.A., Gerber, S.A. & Gygi, S.P. Proc. Natl. Acad. Sci. USA 104, 1488–1493 (2007).

    Article  CAS  Google Scholar 

  4. Pan, C., Olsen, J.V., Daub, H. & Mann, M. Mol. Cell. Proteomics published online, doi: 10.1074/mcp.M900285-MCP200 (August 3, 2009).

  5. Ubersax, J.A. & Ferrell, J.E. Jr. Nat. Rev. Mol. Cell Biol. 8, 530–541 (2007).

    Article  CAS  Google Scholar 

  6. Linding, R. et al. Cell 129, 1415–1426 (2007).

    Article  CAS  Google Scholar 

  7. Van Hoof, D. et al. Cell Stem Cell 5, 214–226 (2009).

    Article  CAS  Google Scholar 

  8. Olsen, J.V. et al. Cell 127, 635–648 (2006).

    Article  CAS  Google Scholar 

  9. Houseman, B.T., Huh, J.H., Kron, S.J. & Mrksich, M. Nat. Biotechnol. 20, 270–274 (2002).

    Article  CAS  Google Scholar 

  10. Lemeer, S. et al. PLoS ONE 2, e581 (2007).

    Article  Google Scholar 

  11. Picotti, P., Bodenmiller, B., Mueller, L.N., Domon, B. & Aebersold, R. Cell 138, 795–806 (2009).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Javier Muñoz and Albert J.R. Heck are in the Biomolecular Mass Spectrometry and Proteomics Group, Utrecht Institute for Pharmaceutical Sciences and Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands.,

    Javier Muñoz & Albert J R Heck

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  1. Javier Muñoz
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  2. Albert J R Heck
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Correspondence to Albert J R Heck.

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Muñoz, J., Heck, A. Snapshots of kinase activities. Nat Biotechnol 27, 912–913 (2009). https://doi.org/10.1038/nbt1009-912

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