Abstract
The multi-copy plasmid vector pIJ702 has been used to clone Streptomyces clavuligerus chromosomal DNA fragments into a clavulanic acid non-producing mutant strain. Two DNA segments, which restored the ability to synthesise clavulanic acid, were shown to overlap one another and share a common sequence of about 2 kb. A preliminary restriction map of the region of the chromosome involved has been constructed.
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Bailey, C., Butler, M., Normansell, I. et al. Cloning A Streptomyces clavuligerus Genetic Locus Involved in Clavulanic Acid Biosynthesis. Nat Biotechnol 2, 808–811 (1984). https://doi.org/10.1038/nbt0984-808
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DOI: https://doi.org/10.1038/nbt0984-808